Literature DB >> 16696068

Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: improvement of sensitivity and fidelity.

L T Wang1, A Smith, B Iacopetta, D J Wood, J M Papadimitriou, M H Zheng.   

Abstract

DNA extraction and PCR amplification from paraffin wax embedded bone tumour specimens present several difficulties, firstly, because of the abundant matrix they contain and, secondly, because decalcification often causes degradation of DNA. In this report, comparative studies were carried out to determine the most efficient method for DNA extraction and PCR amplification from such specimens. The results indicated that nested PCR produced appropriate strong reaction products with minimal background contamination. A method for DNA extraction from paraffin wax embedded bone tissue and a nested PCR-SSCP technique have been developed for use in such diagnostic specimens.

Entities:  

Year:  1996        PMID： 16696068      PMCID： PMC408046          DOI： 10.1136/mp.49.3.m176

Source DB:  PubMed          Journal:  Clin Mol Pathol        ISSN： 1355-2910


  8 in total

1.  Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.

Authors:  R S Cha; H Zarbl; P Keohavong; W G Thilly
Journal:  PCR Methods Appl       Date:  1992-08

2.  Fast and sensitive silver staining of DNA in polyacrylamide gels.

Authors:  B J Bassam; G Caetano-Anollés; P M Gresshoff
Journal:  Anal Biochem       Date:  1991-07       Impact factor: 3.365

3.  Nested polymerase chain reaction assay for the detection of cytomegalovirus overcomes false positives caused by contamination with fragmented DNA.

Authors:  K Porter-Jordan; E I Rosenberg; J F Keiser; J D Gross; A M Ross; S Nasim; C T Garrett
Journal:  J Med Virol       Date:  1990-02       Impact factor: 2.327

4.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Authors:  K B Mullis; F A Faloona
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues.

Authors:  M Koshiba; K Ogawa; S Hamazaki; T Sugiyama; O Ogawa; T Kitajima
Journal:  Pathol Res Pract       Date:  1993-02       Impact factor: 3.250

Review 6.  The p53 tumour suppressor gene.

Authors:  A J Levine; J Momand; C A Finlay
Journal:  Nature       Date:  1991-06-06       Impact factor: 49.962

7.  p53 gene mutation and expression in naevi and melanomas.

Authors:  L E Sparrow; R Soong; H J Dawkins; B J Iacopetta; P J Heenan
Journal:  Melanoma Res       Date:  1995-04       Impact factor: 3.599

Review 8.  The clinical significance of p53 aberrations in human tumours.

Authors:  S Bosari; G Viale
Journal:  Virchows Arch       Date:  1995       Impact factor: 4.064
  8 in total
  5 in total

1.  Frequent Ki-ras mutations and transforming growth factor-alpha expression in adenocarcinomas of the small intestine: report of 7 cases.

Authors:  Hiroyuki Mitomi; Takatoshi Nakamura; Atsushi Ihara; Yoshimasa Otani; Miwa Sada; Masahiro Igarashi; Fumiyuki Akino; Masaaki Ichinoe; Tatsuya Ojima; Akio Mori; Isao Okayasu
Journal:  Dig Dis Sci       Date:  2003-01       Impact factor: 3.199

2.  A pressure cooking-based DNA extraction from archival formalin-fixed, paraffin-embedded tissue.

Authors:  Joon-Yong Chung; Joo Mi Yi; Ran Xie; Victoria Brown; Olivia Lee; Nita Ahuja; Till Braunschweig; Stephen M Hewitt
Journal:  Anal Biochem       Date:  2012-03-23       Impact factor: 3.365

3.  Tumoural specimens for forensic purposes: comparison of genetic alterations in frozen and formalin-fixed paraffin-embedded tissues.

Authors:  Viviana Ananian; Pamela Tozzo; Elena Ponzano; Donato Nitti; Daniele Rodriguez; Luciana Caenazzo
Journal:  Int J Legal Med       Date:  2010-04-06       Impact factor: 2.686

4.  Screening feature genes of osteosarcoma with DNA microarray: a bioinformatic analysis.

Authors:  Yunpeng Zhang; Shi Yan; Daifeng Lu; Feng Dong; Yongyun Lian
Journal:  Int J Clin Exp Med       Date:  2015-05-15

5.  A method to evaluate genome-wide methylation in archival formalin-fixed, paraffin-embedded ovarian epithelial cells.

Authors:  Qiling Li; Min Li; Li Ma; Wenzhi Li; Xuehong Wu; Jendai Richards; Guoxing Fu; Wei Xu; Tameka Bythwood; Xu Li; Jianxin Wang; Qing Song
Journal:  PLoS One       Date:  2014-08-18       Impact factor: 3.240
  5 in total

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