Literature DB >> 1668718

Stereospecific assignment of beta-methylene protons in larger proteins using 3D 15N-separated Hartmann-Hahn and 13C-separated rotating frame Overhauser spectroscopy.

G M Clore1, A Bax, A M Gronenborn.   

Abstract

3J alpha beta coupling constants and complementary nuclear Overhauser data on the intraresidue C alpha H-C beta H distances form an essential part of the data needed to obtain stereospecific assignments of beta-methylene protons in proteins. In this paper we show that information regarding the magnitude of the 3J alpha beta coupling constants can be extracted from a semi-quantitative interpretation of relative peak intensities in a 3D 15N-separated 1H-1H Hartmann-Hahn 1H-15N multiple quantum coherence (HOHAHA-HMQC) spectrum. In addition, we demonstrate that reliable information on the intraresidue C alpha H-C beta H distances, free of systematic errors arising from spin diffusion, can be obtained from a 3D 13C-separated 1H-1H rotating frame Overhauser effect 1H-13C multiple quantum coherence (ROESY-HMQC) spectrum. The applicability of these experiments to larger proteins is illustrated with respect to interleukin-1 beta, a protein of 153 residues and 17.4 kDa molecular weight.

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Year:  1991        PMID： 1668718     DOI： 10.1007/bf01874566

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  22 in total

1.  Assignment of the side-chain 1H and 13C resonances of interleukin-1 beta using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy.

Authors:  G M Clore; A Bax; P C Driscoll; P T Wingfield; A M Gronenborn
Journal:  Biochemistry       Date:  1990-09-04       Impact factor: 3.162

2.  The structure of the Antennapedia homeodomain determined by NMR spectroscopy in solution: comparison with prokaryotic repressors.

Authors:  Y Q Qian; M Billeter; G Otting; M Müller; W J Gehring; K Wüthrich
Journal:  Cell       Date:  1989-11-03       Impact factor: 41.582

3.  A 1H-NMR study of human interleukin-1 beta. Sequence-specific assignment of aromatic residues using site-directed mutant proteins.

Authors:  A M Gronenborn; G M Clore; U Schmeissner; P Wingfield
Journal:  Eur J Biochem       Date:  1986-11-17

4.  Protein structures in solution by nuclear magnetic resonance and distance geometry. The polypeptide fold of the basic pancreatic trypsin inhibitor determined using two different algorithms, DISGEO and DISMAN.

Authors:  G Wagner; W Braun; T F Havel; T Schaumann; N Go; K Wüthrich
Journal:  J Mol Biol       Date:  1987-08-05       Impact factor: 5.469

5.  Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes.

Authors:  J W Ponder; F M Richards
Journal:  J Mol Biol       Date:  1987-02-20       Impact factor: 5.469

6.  Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

Authors:  D Marion; P C Driscoll; L E Kay; P T Wingfield; A Bax; A M Gronenborn; G M Clore
Journal:  Biochemistry       Date:  1989-07-25       Impact factor: 3.162

7.  Identification and localization of bound internal water in the solution structure of interleukin 1 beta by heteronuclear three-dimensional 1H rotating-frame Overhauser 15N-1H multiple quantum coherence NMR spectroscopy.

Authors:  G M Clore; A Bax; P T Wingfield; A M Gronenborn
Journal:  Biochemistry       Date:  1990-06-19       Impact factor: 3.162

8.  High-resolution three-dimensional structure of interleukin 1 beta in solution by three- and four-dimensional nuclear magnetic resonance spectroscopy.

Authors:  G M Clore; P T Wingfield; A M Gronenborn
Journal:  Biochemistry       Date:  1991-03-05       Impact factor: 3.162

9.  Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing.

Authors:  J Kraulis; G M Clore; M Nilges; T A Jones; G Pettersson; J Knowles; A M Gronenborn
Journal:  Biochemistry       Date:  1989-09-05       Impact factor: 3.162

10.  Determination of the secondary structure and molecular topology of interleukin-1 beta by use of two- and three-dimensional heteronuclear 15N-1H NMR spectroscopy.

Authors:  P C Driscoll; A M Gronenborn; P T Wingfield; G M Clore
Journal:  Biochemistry       Date:  1990-05-15       Impact factor: 3.162
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  21 in total

1.  HYPER: a hierarchical algorithm for automatic determination of protein dihedral-angle constraints and stereospecific C beta H2 resonance assignments from NMR data.

Authors:  R Tejero; D Monleon; B Celda; R Powers; G T Montelione
Journal:  J Biomol NMR       Date:  1999-11       Impact factor: 2.835

2.  Nuclear overhauser spectroscopy of chiral CHD methylene groups.

Authors:  Rafal Augustyniak; Jan Stanek; Henri Colaux; Geoffrey Bodenhausen; Wiktor Koźmiński; Torsten Herrmann; Fabien Ferrage
Journal:  J Biomol NMR       Date:  2015-11-27       Impact factor: 2.835

3.  Sortase-assembled pili in Corynebacterium diphtheriae are built using a latch mechanism.

Authors:  Scott A McConnell; Rachel A McAllister; Brendan R Amer; Brendan J Mahoney; Christopher K Sue; Chungyu Chang; Hung Ton-That; Robert T Clubb
Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-23       Impact factor: 11.205

4.  Chi 1 angle information from a simple two-dimensional NMR experiment that identifies trans 3JNC gamma couplings in isotopically enriched proteins.

Authors:  J S Hu; A Bax
Journal:  J Biomol NMR       Date:  1997-04       Impact factor: 2.835

5.  Carboxyl pK(a) values, ion pairs, hydrogen bonding, and the pH-dependence of folding the hyperthermophile proteins Sac7d and Sso7d.

Authors:  Andrew T Clark; Kelley Smith; Ranjith Muhandiram; Stephen P Edmondson; John W Shriver
Journal:  J Mol Biol       Date:  2007-07-10       Impact factor: 5.469

6.  The solution structure of the DNA-binding domain of Skn-1.

Authors:  M C Lo; S Ha; I Pelczer; S Pal; S Walker
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-21       Impact factor: 11.205

7.  Three-dimensional solution structure of the HIV-1 protease complexed with DMP323, a novel cyclic urea-type inhibitor, determined by nuclear magnetic resonance spectroscopy.

Authors:  T Yamazaki; A P Hinck; Y X Wang; L K Nicholson; D A Torchia; P Wingfield; S J Stahl; J D Kaufman; C H Chang; P J Domaille; P Y Lam
Journal:  Protein Sci       Date:  1996-03       Impact factor: 6.725

8.  Refined structure of villin 14T and a detailed comparison with other actin-severing domains.

Authors:  M A Markus; P Matsudaira; G Wagner
Journal:  Protein Sci       Date:  1997-06       Impact factor: 6.725

9.  The influence of C-terminal extension on the structure of the "J-domain" in E. coli DnaJ.

Authors:  K Huang; J M Flanagan; J H Prestegard
Journal:  Protein Sci       Date:  1999-01       Impact factor: 6.725

10.  Structure of the Bacillus anthracis Sortase A Enzyme Bound to Its Sorting Signal: A FLEXIBLE AMINO-TERMINAL APPENDAGE MODULATES SUBSTRATE ACCESS.

Authors:  Albert H Chan; Sung Wook Yi; Austen L Terwilliger; Anthony W Maresso; Michael E Jung; Robert T Clubb
Journal:  J Biol Chem       Date:  2015-08-31       Impact factor: 5.157
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