Literature DB >> 16682482

A simple manual rosetting method for absolute CD4+ lymphocyte counting in resource-limited countries.

Phonethipsavanh Nouanthong1, Supansa Pata, Thira Sirisanthana, Watchara Kasinrerk.   

Abstract

A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.

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Year:  2006        PMID: 16682482      PMCID: PMC1459645          DOI: 10.1128/CVI.13.5.598-601.2006

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN: 1556-679X


  13 in total

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3.  Statistical methods for assessing agreement between two methods of clinical measurement.

Authors:  J M Bland; D G Altman
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4.  Report of a WHO workshop on flow cytometry and alternative methodologies for CD4 lymphocyte determinations: applications for developing countries Washington, DC, 16 April 1992. World Health Organization Global Programme on AIDS.

Authors: 
Journal:  AIDS       Date:  1994-01       Impact factor: 4.177

5.  Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay.

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Journal:  Clin Diagn Lab Immunol       Date:  1995-05

6.  An inexpensive, simple, and manual method of CD4 T-cell quantitation in HIV-infected individuals for use in developing countries.

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7.  A manual bead assay for the determination of absolute CD4+ and CD8+ lymphocyte counts in human immunodeficiency virus-infected individuals.

Authors:  A V Carella; M W Moss; V Provost; T C Quinn
Journal:  Clin Diagn Lab Immunol       Date:  1995-09

8.  Evaluation of alternative CD4 technologies for the enumeration of CD4 lymphocytes.

Authors:  J K Nicholson; W M Velleca; S Jubert; T A Green; L Bryan
Journal:  J Immunol Methods       Date:  1994-12-28       Impact factor: 2.303

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Journal:  Int J Hematol       Date:  2010-08-26       Impact factor: 2.490

2.  Finger-prick blood samples can be used interchangeably with venous samples for CD4 cell counting indicating their potential for use in CD4 rapid tests.

Authors:  Calman A MacLennan; Joep J G van Oosterhout; Sarah A White; Mark T Drayson; Eduard E Zijlstra; Malcolm E Molyneux
Journal:  AIDS       Date:  2007-07-31       Impact factor: 4.177

3.  Diagnostic accuracy and clinical utility of a simplified low cost method of counting CD4 cells with flow cytometry in Malawi: diagnostic accuracy study.

Authors:  Calman A MacLennan; Michael K P Liu; Sarah A White; Joep J G van Oosterhout; Felanji Simukonda; Joseph Bwanali; Michael J Moore; Eduard E Zijlstra; Mark T Drayson; Malcolm E Molyneux
Journal:  BMJ       Date:  2007-07-17
  3 in total

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