Literature DB >> 16665902

Glucosinolate Biosynthesis: Sulfation of Desulfobenzylglucosinolate by Cell-Free Extracts of Cress (Lepidium sativum L.) Seedlings.

T M Glendening1, J E Poulton.   

Abstract

After removal of myrosinase activity by concanavalin A-Sepharose 4B chromatography, cell-free extracts of light-grown cress (Lepidium sativum L.) seedlings, catalyzed the sulfation of desulfobenzylglucosinolate (K(m), 0.23 millimolar) to benzylglucosinolate using PAPS (K(m), 1 millimolar) as sulfur donor. Sulfotransferase activity, which was optimal at pH 9.0, was stimulated by MgCl(2), MnCl(2), beta-mercaptoethanol, and dithiothreitol and was inhibited by ZnSO(4) and SH-reagents. The enzyme also sulfated desulfoallyglucosinolate to allylglucosinolate (sinigrin) but was inactive towards all phenylpropanoids and flavonoids tested.

Entities:  

Year:  1988        PMID： 16665902      PMCID： PMC1054477          DOI： 10.1104/pp.86.2.319

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  9 in total

1.  Biosynthesis of mustard oil glucosides. I. Administration of C14-labelled compounds to horseradish, nasturtium, and watercress.

Authors:  E W UNDERHILL; M D CHISHOLM; L R WETTER
Journal:  Can J Biochem Physiol       Date:  1962-11

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Biosynthesis of glucosinolates.

Authors:  E W Underhill; L R Wetter; M D Chisholm
Journal:  Biochem Soc Symp       Date:  1973

4.  Sources of sulfur in the thioglucosides of various higher plants.

Authors:  L R Wetter; M D Chisholm
Journal:  Can J Biochem       Date:  1968-08

5.  The isolation and purification of indole glucosinolates from Brassica species.

Authors:  R J Truscott; I Minchinton; J Sang
Journal:  J Sci Food Agric       Date:  1983-03       Impact factor: 3.638

6.  Biosynthesis of Mustard Oil Glucosides: Sodium Phenylacetothiohydroximate and Desulfobenzylglucosinolate, Precursors of Benzylglucosinolate in Tropaeolum majus.

Authors:  L E Underhill; L R Wetter
Journal:  Plant Physiol       Date:  1969-04       Impact factor: 8.340

7.  An intermediate in the synthesis of glucobrassicins from 3-indoleacetaldoxime by woad leaves.

Authors:  S Mahadevan; B B Stowe
Journal:  Plant Physiol       Date:  1972-07       Impact factor: 8.340

8.  Biosynthesis of mustard oil glucosides: conversion of phenylacetaldehyde oxime and 3-phenylpropionaldehyde oxime to glucotropaeolin and gluconasturtiin.

Authors:  E W Underhill
Journal:  Eur J Biochem       Date:  1967-07

9.  On the mechanism of aryl sulfotransferase.

Authors:  M W Duffel; W B Jakoby
Journal:  J Biol Chem       Date:  1981-11-10       Impact factor: 5.157
  9 in total
  10 in total

1.  Effect of Castanospermine and Related Polyhydroxyalkaloids on Purified Myrosinase from Lepidium sativum Seedlings.

Authors:  P L Durham; J E Poulton
Journal:  Plant Physiol       Date:  1989-05       Impact factor: 8.340

2.  Partial Purification and Characterization of Three Flavonol-Specific Sulfotransferases from Flaveria chloraefolia.

Authors:  L Varin; R K Ibrahim
Journal:  Plant Physiol       Date:  1989-07       Impact factor: 8.340

3.  Partial Purification and Characterization of a 3'- Phosphoadenosine 5' -Phosphosulfate: Desulfoglucosinolate Sulfotransferase from Cress (Lepidium sativum).

Authors:  T M Glendening; J E Poulton
Journal:  Plant Physiol       Date:  1990-10       Impact factor: 8.340

4.  Expression of Mammalian Metallothionein Suppresses Glucosinolate Synthesis in Brassica campestris.

Authors:  D D Lefebvre
Journal:  Plant Physiol       Date:  1990-06       Impact factor: 8.340

5.  An unusual case of 'uncompetitive activation' by ascorbic acid: purification and kinetic properties of a myrosinase from Raphanus sativus seedlings.

Authors:  M Shikita; J W Fahey; T R Golden; W D Holtzclaw; P Talalay
Journal:  Biochem J       Date:  1999-08-01       Impact factor: 3.857

6.  Immunopurification and Immunocharacterization of the Glucosinolate Biosynthetic Enzyme Thiohydroximate S-Glucosyltransferase.

Authors:  JWD. GrootWassink; D. W. Reed; A. D. Kolenovsky
Journal:  Plant Physiol       Date:  1994-05       Impact factor: 8.340

7.  Synthesis of Benzylglucosinolate in Tropaeolum majus L. (Isothiocyanates as Potent Enzyme Inhibitors).

Authors:  J. Lykkesfeldt; B. L. Moller
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

8.  Isolation of a Microsomal Enzyme System Involved in Glucosinolate Biosynthesis from Seedlings of Tropaeolum majus L.

Authors:  L. Du; B. A. Halkier
Journal:  Plant Physiol       Date:  1996-07       Impact factor: 8.340

9.  Molecular cloning of a sulfotransferase in Arabidopsis thaliana and regulation during development and in response to infection with pathogenic bacteria.

Authors:  C Lacomme; D Roby
Journal:  Plant Mol Biol       Date:  1996-03       Impact factor: 4.076

10.  Involvement of cytochrome P450 in oxime production in glucosinolate biosynthesis as demonstrated by an in vitro microsomal enzyme system isolated from jasmonic acid-induced seedlings of Sinapis alba L.

Authors:  L Du; J Lykkesfeldt; C E Olsen; B A Halkier
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-19       Impact factor: 11.205
  10 in total

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