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Literature DB >> 16665823

Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains.

I Dupuis¹, P Roeckel, E Matthys-Rochon, C Dumas.

Abstract

Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells which lack cell wall and plastids, and can be considered as haploid protoplasts.

Entities: Chemical Species

Year: 1987 PMID： 16665823 PMCID： PMC1054361 DOI： 10.1104/pp.85.4.876

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

6 in total

1. Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.

Authors: S D Russell
Journal: Plant Physiol Date: 1986-05 Impact factor: 8.340

2. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate.

Authors: J Heslop-Harrison; Y Heslop-Harrison
Journal: Stain Technol Date: 1970-05

3. Isolation of vegetative and generative nuclei from pollen tubes.

Authors: K L LaFountain; J P Mascarenhas
Journal: Exp Cell Res Date: 1972-07 Impact factor: 3.905

Review 4. Elimination of plastids during spermatogenesis and fertilization in the plant kingdom.

Authors: B B Sears
Journal: Plasmid Date: 1980-11 Impact factor: 3.466

5. Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm.

Authors: S D Russell
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

6. Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum.

Authors: E Matthys-Rochon; P Vergne; S Detchepare; C Dumas
Journal: Plant Physiol Date: 1987-03 Impact factor: 8.340

6 in total

14 in total

1. Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants.

Authors: C. Dumas; H. L. Mogensen
Journal: Plant Cell Date: 1993-10 Impact factor: 11.277

2. Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

Authors: N R Geltz; S D Russell
Journal: Plant Physiol Date: 1988-11 Impact factor: 8.340

3. Zygotic Genome Activation Occurs Shortly after Fertilization in Maize.

Authors: Junyi Chen; Nicholas Strieder; Nadia G Krohn; Philipp Cyprys; Stefanie Sprunck; Julia C Engelmann; Thomas Dresselhaus
Journal: Plant Cell Date: 2017-08-16 Impact factor: 11.277

4. Influence of Temperature Stress on in Vitro Fertilization and Heat Shock Protein Synthesis in Maize (Zea mays L.) Reproductive Tissues.

Authors: I Dupuis; C Dumas
Journal: Plant Physiol Date: 1990-10 Impact factor: 8.340

10. Karyogamy after Electrofusion of Single Egg and Sperm Cell Protoplasts from Maize: Cytological Evidence and Time Course.

Authors: J. E. Faure; H. L. Mogensen; C. Dumas; H. Lorz; E. Kranz
Journal: Plant Cell Date: 1993-07 Impact factor: 11.277