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Literature DB >> 16665270

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum.

E Matthys-Rochon¹, P Vergne, S Detchepare, C Dumas.

Abstract

A technical procedure is described to follow the in vitro release of the ;male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been checked by the fluorochromatic reaction test. These preliminary results indicate that this procedure appears to be a prerequisite for the successful preparation of purified viable sperm cells.

Entities: Species

Year: 1987 PMID： 16665270 PMCID： PMC1056385 DOI： 10.1104/pp.83.3.464

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

12 in total

1. Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific aniline blue fluorochrome.

Authors: T Hough; P Bernhardt; R B Knox; E G Williams
Journal: Stain Technol Date: 1985-05

2. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.

Authors: K H Jones; J A Senft
Journal: J Histochem Cytochem Date: 1985-01 Impact factor: 2.479

3. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.

Authors: J M Widholm
Journal: Stain Technol Date: 1972-07

4. Evaluation of pollen viability by enzymatically induced fluorescence; intracellular hydrolysis of fluorescein diacetate.

Authors: J Heslop-Harrison; Y Heslop-Harrison
Journal: Stain Technol Date: 1970-05

5. Isolation of vegetative and generative nuclei from pollen tubes.

Authors: K L LaFountain; J P Mascarenhas
Journal: Exp Cell Res Date: 1972-07 Impact factor: 3.905

6. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

Authors: B Rotman; B W Papermaster
Journal: Proc Natl Acad Sci U S A Date: 1966-01 Impact factor: 11.205

Review 7. Cellular nucleotide measurements and applications in microbial ecology.

Authors: D M Karl
Journal: Microbiol Rev Date: 1980-12

8. Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm.

Authors: S D Russell
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

9. Adenosine triphosphate in human semen: a quantitative estimate of fertilizing potential.

Authors: F Comhaire; L Vermeulen; K Ghedira; J Mas; S Irvine; G Callipolitis
Journal: Fertil Steril Date: 1983-10 Impact factor: 7.329

10. Applications of fluorochromes to pollen biology. I. Mithramycin and 4',6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA.

Authors: A W Coleman; L J Goff
Journal: Stain Technol Date: 1985-05

6 in total

1. Gametes and Fertilization: Maize as a Model System for Experimental Embryogenesis in Flowering Plants.

Authors: C. Dumas; H. L. Mogensen
Journal: Plant Cell Date: 1993-10 Impact factor: 11.277

2. Isolation of viable wheat male gametophytes of different stages of development and variations in their protein patterns.

Authors: P Vergne; C Dumas
Journal: Plant Physiol Date: 1988-12 Impact factor: 8.340