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Literature DB >> 16664799

Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.

Abstract

Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.

Entities: Chemical Disease Species

Year: 1986 PMID： 16664799 PMCID： PMC1075328 DOI： 10.1104/pp.81.1.317

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

4 in total

1. Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific aniline blue fluorochrome.

Authors: T Hough; P Bernhardt; R B Knox; E G Williams
Journal: Stain Technol Date: 1985-05

2. Isolation of vegetative and generative nuclei from pollen tubes.

Authors: K L LaFountain; J P Mascarenhas
Journal: Exp Cell Res Date: 1972-07 Impact factor: 3.905

3. Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm.

Authors: S D Russell
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

4. Pollen sporoplasts: dissolution of pollen walls.

Authors: F A Loewus; B G Baldi; V R Franceschi; L D Meinert; J J McCollum
Journal: Plant Physiol Date: 1985-07 Impact factor: 8.340

4 in total

12 in total

1. Isolation of Fixed and Viable Eggs, Central Cells, and Embryo Sacs from Ovules of Plumbago zeylanica.

Authors: B Q Huang; S D Russell
Journal: Plant Physiol Date: 1989-05 Impact factor: 8.340

2. Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

Authors: N R Geltz; S D Russell
Journal: Plant Physiol Date: 1988-11 Impact factor: 8.340

10. Methods to isolate a large amount of generative cells, sperm cells and vegetative nuclei from tomato pollen for "omics" analysis.

Authors: Yunlong Lu; Liqin Wei; Tai Wang
Journal: Front Plant Sci Date: 2015-06-02 Impact factor: 5.753

Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.

1. Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific aniline blue fluorochrome.

2. Isolation of vegetative and generative nuclei from pollen tubes.

3. Preferential fertilization in Plumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm.

4. Pollen sporoplasts: dissolution of pollen walls.

1. Isolation of Fixed and Viable Eggs, Central Cells, and Embryo Sacs from Ovules of Plumbago zeylanica.

2. Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

3. Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains.

4. Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization.

5. Flow Cytometric Characteristics of Sperm Cells Isolated from Pollen of Zea mays L.

6. Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum.

7. An efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in Brassica napus pollen.

8. Improvement of Longevity and Viability of Sperm Cells Isolated from Pollen of Zea mays L.

9. Isolation and purification of generative cells from fresh pollen of Vicia faba L.

10. Methods to isolate a large amount of generative cells, sperm cells and vegetative nuclei from tomato pollen for "omics" analysis.