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Literature DB >> 16665144

Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts.

Abstract

Vacuoles isolated from pea (Pisum sativum), and wheat (Triticum aestivum) leaf protoplasts contained considerable activities of electrophoretically highly mobile exoamylases. Vacuoles from spinach (Spinacia oleracea) leaf and photoautotrophic Chenopodium rubrum suspension culture cell protoplasts were devoid of amylolytic activity. Endoamylase activity was in all cases associated primarily with the chloroplast.

Entities: Species

Year: 1986 PMID： 16665144 PMCID： PMC1056268 DOI： 10.1104/pp.82.4.1119

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

9 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

Authors: D I Arnon
Journal: Plant Physiol Date: 1949-01 Impact factor: 8.340

3. The hydrolysis of maltodextrins by a -amylase isolated from leaves of Vicia faba.

Authors: G W Chapman; J E Pallas; J Mendicino
Journal: Biochim Biophys Acta Date: 1972-08-28

4. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors: J J Sedmak; S E Grossberg
Journal: Anal Biochem Date: 1977-05-01 Impact factor: 3.365

5. Hydrolytic enzymes in the central vacuole of plant cells.

Authors: T Boller; H Kende
Journal: Plant Physiol Date: 1979-06 Impact factor: 8.340

6. Starch Degradation in Spinach Leaves: ISOLATION AND CHARACTERIZATION OF THE AMYLASES AND R-ENZYME OF SPINACH LEAVES.

Authors: T W Okita; J Preiss
Journal: Plant Physiol Date: 1980-11 Impact factor: 8.340

7. Electrophoretic transfer as a technique for the detection and identification of plant amylolytic enzymes in polyacrylamide gels.

Authors: G Kakefuda; S H Duke
Journal: Plant Physiol Date: 1984-05 Impact factor: 8.340

8. Development of Nitrogen Assimilation Enzymes during Photoautotrophic Growth of Chenopodium rubrum Suspension Cultures.

Authors: W H Campbell; P Ziegler; E Beck
Journal: Plant Physiol Date: 1984-04 Impact factor: 8.340

9. Water stress enhances expression of an alpha-amylase gene in barley leaves.

Authors: J V Jacobsen; A D Hanson; P C Chandler
Journal: Plant Physiol Date: 1986-02 Impact factor: 8.340

9 in total

26 in total

9. Starch Degradation and Distribution of the Starch-Degrading Enzymes in Vicia faba Leaves (Diurnal Oscillation of Amylolytic Activity and Starch Content in Chloroplasts).

Authors: C. Ghiena; M. Schulz; H. Schnabl
Journal: Plant Physiol Date: 1993-01 Impact factor: 8.340

10. Light-Induced Chloroplast [alpha]-Amylase in Pearl Millet (Pennisetum americanum).

Authors: KJM. Vally; R. Sharma
Journal: Plant Physiol Date: 1995-02 Impact factor: 8.340

Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

3. The hydrolysis of maltodextrins by a -amylase isolated from leaves of Vicia faba.

4. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

5. Hydrolytic enzymes in the central vacuole of plant cells.

6. Starch Degradation in Spinach Leaves: ISOLATION AND CHARACTERIZATION OF THE AMYLASES AND R-ENZYME OF SPINACH LEAVES.

7. Electrophoretic transfer as a technique for the detection and identification of plant amylolytic enzymes in polyacrylamide gels.

8. Development of Nitrogen Assimilation Enzymes during Photoautotrophic Growth of Chenopodium rubrum Suspension Cultures.

9. Water stress enhances expression of an alpha-amylase gene in barley leaves.

1. The ram1 mutant of Arabidopsis exhibits severely decreased beta-amylase activity.

2. Partial purification and characterization of the major endoamylase of mature pea leaves.

3. Localization of alpha-Amylase in the Apoplast of Pea (Pisum sativum L.) Stems.

4. Subcellular localization and characterization of amylases in Arabidopsis leaf.

5. Nucleotide Sequence of a cDNA Clone Encoding a beta-Amylase from Arabidopsis thaliana.

6. Characterization of alpha-Amylase from Shoots and Cotyledons of Pea (Pisum sativum L.) Seedlings.

7. Amylases in Pea Tissues with Reduced Chloroplast Density and/or Function.

8. Purification of a beta-Amylase that Accumulates in Arabidopsis thaliana Mutants Defective in Starch Metabolism.

9. Starch Degradation and Distribution of the Starch-Degrading Enzymes in Vicia faba Leaves (Diurnal Oscillation of Amylolytic Activity and Starch Content in Chloroplasts).

10. Light-Induced Chloroplast [alpha]-Amylase in Pearl Millet (Pennisetum americanum).