Literature DB >> 16664848

Monoclonal Antibodies to Plant Growth Regulators: III. Zeatinriboside and Dihydrozeatinriboside.

J Eberle1, A Arnscheidt, D Klix, E W Weiler.   

Abstract

Four high affinity monoclonal antibodies, which recognize two plant growth regulators from the cytokinin group, namely trans-zeatin riboside and dihydrozeatin riboside and their derivatives are reported. Six hybridomas were produced from three independent fusions of Balb/c spleen cells with P3-NS1-Ag 4-1 (abbreviated NS1) or X63-Ag 8.653 (X63) myeloma cells. The mice had been hyperimmunized with zeatin riboside-bovine serum albumin conjugate or dihydrozeatin riboside-bovine serum albumin conjugate for 3 months. The hybridomas secrete antibodies of the IgG 1 or IgG 2b subclass and allow the detection of femtomole amounts of the free cytokinins, their ribosides, and ribotides in plant extracts. The use of these monoclonals in radio- and enzyme-linked immunosorbent assay is also discussed.

Entities:  

Year:  1986        PMID： 16664848      PMCID： PMC1075368          DOI： 10.1104/pp.81.2.516

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  12 in total

1.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.

Authors:  J F Kearney; A Radbruch; B Liesegang; K Rajewsky
Journal:  J Immunol       Date:  1979-10       Impact factor: 5.422

2.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

3.  ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA.

Authors:  B F ERLANGER; S M BEISER
Journal:  Proc Natl Acad Sci U S A       Date:  1964-07       Impact factor: 11.205

4.  ZEATIN, A FACTOR INDUCING CELL DIVISION ISOLATED FROM ZEA MAYS.

Authors:  D S LETHAM
Journal:  Life Sci (1962)       Date:  1963-08

5.  Diffusion-in-gel methods for immunological analysis.

Authors:  O OUCHTERLONY
Journal:  Prog Allergy       Date:  1958

6.  Trans-zeatin in culture filtrates of Agrobacterium tumefaciens.

Authors:  R W Kaiss-Chapman; R O Morris
Journal:  Biochem Biophys Res Commun       Date:  1976-05-23       Impact factor: 3.575

7.  Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines.

Authors:  G Köhler; S C Howe; C Milstein
Journal:  Eur J Immunol       Date:  1976-04       Impact factor: 5.532

Review 8.  Antibody engineering, a strategy for the development of monoclonal antibodies.

Authors:  O T Schönherr; E H Houwink
Journal:  Antonie Van Leeuwenhoek       Date:  1984       Impact factor: 2.271

9.  Production of reagent antibodies.

Authors:  B A Hurn; S M Chantler
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

10.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600
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  15 in total

1.  Monoclonal antibodies against fusicoccin with binding characteristics similar to the putative fusicoccin receptor of higher plants.

Authors:  M Feyerabend; E W Weiler
Journal:  Plant Physiol       Date:  1987-11       Impact factor: 8.340

2.  Immunoassay and ultrastructural localization of isopentenyladenine and related cytokinins using monoclonal antibodies.

Authors:  J Eberle; T L Wang; S Cook; B Wells; E W Weiler
Journal:  Planta       Date:  1987-11       Impact factor: 4.116

3.  Relationship between endogenous auxin and cytokinin levels and morphogenic responses inActinidia deliciosa tissue cultures.

Authors:  M L Centeno; A Rodríguez; I Feito; B Fernández
Journal:  Plant Cell Rep       Date:  1996-11       Impact factor: 4.570

4.  Phaseic Acid, an Endogenous and Reversible Inhibitor of Glutamate Receptors in Mouse Brain.

Authors:  Sheng Tao Hou; Susan X Jiang; L Irina Zaharia; Xiumei Han; Chantel L Benson; Jacqueline Slinn; Suzanne R Abrams
Journal:  J Biol Chem       Date:  2016-11-18       Impact factor: 5.157

5.  Hybrid hybridoma cell lines which express anti-zeatin riboside, anti-abscisic Acid, and hybrid antibodies.

Authors:  G M Banowetz
Journal:  Plant Physiol       Date:  1989-09       Impact factor: 8.340

6.  Immunodetection and Identification of N-(o-Hydroxybenzylamino)Purine as a Naturally Occurring Cytokinin in Populus x canadensis Moench cv Robusta Leaves.

Authors:  M Strnad; W Peters; E Beck; M Kamínek
Journal:  Plant Physiol       Date:  1992-05       Impact factor: 8.340

7.  Phenotype and hormonal status of transgenic tobacco plants overexpressing the rolA gene of Agrobacterium rhizogenes T-DNA.

Authors:  C Dehio; K Grossmann; J Schell; T Schmülling
Journal:  Plant Mol Biol       Date:  1993-12       Impact factor: 4.076

8.  Specification of cortical parenchyma and stele of maize primary roots by asymmetric levels of auxin, cytokinin, and cytokinin-regulated proteins.

Authors:  Muhammad Saleem; Tobias Lamkemeyer; André Schützenmeister; Johannes Madlung; Hajime Sakai; Hans-Peter Piepho; Alfred Nordheim; Frank Hochholdinger
Journal:  Plant Physiol       Date:  2009-11-20       Impact factor: 8.340

9.  A robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin signaling network in planta.

Authors:  Evelyne Zürcher; Deborah Tavor-Deslex; Dmytro Lituiev; Katalin Enkerli; Paul T Tarr; Bruno Müller
Journal:  Plant Physiol       Date:  2013-01-25       Impact factor: 8.340

10.  Role of cytokinin in the regulation of root gravitropism.

Authors:  Roni Aloni; Markus Langhans; Erez Aloni; Cornelia I Ullrich
Journal:  Planta       Date:  2004-09-10       Impact factor: 4.116
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