Literature DB >> 1663152

The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes.

C B Faulds1, G Williamson.   

Abstract

A 4-hydroxy-3-methoxycinnamic acid (ferulic acid) esterase has been purified from the extracellular broth of cultures of Streptomyces olivochromogenes after growth on oat splet xylan. The purification procedure utilizes ion exchange on DEAE-BioGel A, anion exchange on Mono Q, gel filtration and hydrophobic interaction chromatography. The purified enzyme appeared as a single band on SDS-PAGE, with an apparent Mr of 29,000. Two bands, at pI7.9 and 8.5, were observed on isoelectric focusing. With methyl ferulate as substrate, the pH and temperature optima were 5.5 and 30 degrees C respectively, with a Km of 1.86 mM and Vmax of 0.3 mumols min-1 mg-1. The purfied enzyme released ferulic acid from de-starched wheat bran only in the presence of xylanase.

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Year:  1991        PMID： 1663152     DOI： 10.1099/00221287-137-10-2339

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  19 in total

1.  A non-modular type B feruloyl esterase from Neurospora crassa exhibits concentration-dependent substrate inhibition.

Authors:  Valerie F Crepin; Craig B Faulds; Ian F Connerton
Journal:  Biochem J       Date:  2003-03-01       Impact factor: 3.857

2.  Purification and properties of a feruloyl esterase involved in lignocellulose degradation by Aureobasidium pullulans.

Authors:  Karl Rumbold; Peter Biely; Maria Mastihubová; Marinka Gudelj; Georg Gübitz; Karl-Heinz Robra; Bernard A Prior
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

3.  Bacterial cinnamoyl esterase activity screening for the production of a novel functional food product.

Authors:  Simone Guglielmetti; Ivano De Noni; Federica Caracciolo; Francesco Molinari; Carlo Parini; Diego Mora
Journal:  Appl Environ Microbiol       Date:  2007-12-28       Impact factor: 4.792

4.  Cloning, expression of a feruloyl esterase from Aspergillus usamii E001 and its applicability in generating ferulic acid from wheat bran.

Authors:  Yan-Yan Gong; Xin Yin; Hui-Min Zhang; Min-Chen Wu; Cun-Duo Tang; Jun-Qing Wang; Qing-Feng Pang
Journal:  J Ind Microbiol Biotechnol       Date:  2013-09-20       Impact factor: 3.346

5.  An Aspergillus niger esterase (ferulic acid esterase III) and a recombinant Pseudomonas fluorescens subsp. cellulosa esterase (Xy1D) release a 5-5' ferulic dehydrodimer (diferulic acid) from barley and wheat cell walls.

Authors:  B Bartolomé; C B Faulds; P A Kroon; K Waldron; H J Gilbert; G Hazlewood; G Williamson
Journal:  Appl Environ Microbiol       Date:  1997-01       Impact factor: 4.792

6.  Characterization of Cinnamoyl Esterases from Different Lactobacilli and Bifidobacteria.

Authors:  Caroline Fritsch; André Jänsch; Matthias A Ehrmann; Simone Toelstede; Rudi F Vogel
Journal:  Curr Microbiol       Date:  2016-12-21       Impact factor: 2.188

7.  Measurement of feruloyl/p-coumaroyl esterase by capillary zone electrophoresis.

Authors:  J A Donaghy; A M McKay
Journal:  World J Microbiol Biotechnol       Date:  1995-03       Impact factor: 3.312

8.  An Aspergillus awamori acetylesterase: purification of the enzyme, and cloning and sequencing of the gene.

Authors:  T Koseki; S Furuse; K Iwano; H Sakai; H Matsuzawa
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

9.  Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger.

Authors:  C B Faulds; G Williamson
Journal:  Appl Microbiol Biotechnol       Date:  1995-11       Impact factor: 4.813

10.  Purification and some characteristics of the acetylxylan esterase from Schizophyllum commune.

Authors:  N Halgasová; E Kutejová; J Timko
Journal:  Biochem J       Date:  1994-03-15       Impact factor: 3.857
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