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Literature DB >> 1660147

A contingent replication assay for the detection of protein-protein interactions in animal cells.

H A Vasavada¹, S Ganguly, F J Germino, Z X Wang, S M Weissman.

Abstract

We have developed a sensitive and rapid assay system termed the contingent replication assay (CRA) for selecting cDNAs with desired functional properties from a cDNA library. The system functions in animal cells and permits enrichment of the desired cDNA in small-scale and convenient experiments. The assay can be used for the enrichment of proteins that activate transcription from conditional enhancers, bind to specific DNA sequences, or interact with target proteins of interest. In this communication we report the application of this assay to study protein-protein interactions in animal cells.

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Year: 1991 PMID： 1660147 PMCID： PMC52995 DOI： 10.1073/pnas.88.23.10686

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

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6. A model system for the rescue of cDNA encoding transacting transcription activator by contingent replication assay.

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8. Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.

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9. Evaluation of the contingent replication assay (CRA) and its application to the study of the general transcription initiation factor, TFIIF.

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10. Domain structure of a human general transcription initiation factor, TFIIF.

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