| Literature DB >> 16594659 |
Rachael M Crist1, Chrysoula Vasileiou, Montserrat Rabago-Smith, James H Geiger, Babak Borhan.
Abstract
Due to the difficulties in handling and manipulating membrane-bound proteins, such as rhodopsin, and the lack of crystallographic information on the cone opsins, we have opted to engineer a protein mimic of the transmembrane G-protein coupled receptor. Human cellular retinoic acid binding protein (CRABPII), a well studied and characterized protein, has been reengineered into a protein that now will bind retinal as a protonated Schiff base with high binding affinity (Kd = 2 nM) mimicking that of rhodopsin.Entities:
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Year: 2006 PMID： 16594659 DOI： 10.1021/ja058591m
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419