Literature DB >> 1655706

Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA.

J Madoń1, R Hütter.   

Abstract

A new procedure for transformation of Amycolatopsis (Nocardia) mediterranei LBG A3136 was developed. The method makes use of polyethylene glycol and alkaline cations and enables direct transformation of the A. mediterranei mycelium with high efficiency: more than 10(6) transformants per microgram of DNA were obtained. Transformation of A. mediterranei is stimulated by the ionophore antibiotic valinomycin and abolished by arsenate and p-chloromercuribenzenesulfonate. pMEA123, a vector based on the indigenous plasmid pMEA100 and containing the erythromycin resistance gene, was constructed.

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Year:  1991        PMID： 1655706      PMCID： PMC208963          DOI： 10.1128/jb.173.20.6325-6331.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Site-specific integration and excision of pMEA100 in Nocardia mediterranei.

Authors:  J Madon; P Moretti; R Hütter
Journal:  Mol Gen Genet       Date:  1987-09

2.  Mechanisms of active transport in isolated bacterial membrane vesicles. 8. Valinomycin-induced rubidium transport.

Authors:  F J Lombardi; J P Reeves; H R Kaback
Journal:  J Biol Chem       Date:  1973-05-25       Impact factor: 5.157

3.  Energization of active transport by Escherichia coli.

Authors:  W L Klein; P D Boyer
Journal:  J Biol Chem       Date:  1972-11-25       Impact factor: 5.157

4.  Physico-chemical basis of ion transport through biological membranes: ionophores and ion channels.

Authors:  Y A Ovchinnikov
Journal:  Eur J Biochem       Date:  1979-03

5.  Amplification on the Amycolatopsis (Nocardia) mediterranei plasmid pMEA100: sequence similarities to actinomycete att sites.

Authors:  B Zhu; J Madoń; A Häusler; R Hütter
Journal:  Plasmid       Date:  1990-09       Impact factor: 3.466

6.  Valinomycin-induced uptake of potassium in membrane vesicles from Escherichia coli.

Authors:  P Bhattacharyya; W Epstein; S Silver
Journal:  Proc Natl Acad Sci U S A       Date:  1971-07       Impact factor: 11.205

7.  Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells.

Authors:  A G Sabelnikov; I V Domaradsky
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

8.  Isolation and characterization of an extrachromosomal element from Nocardia mediterranei.

Authors:  P Moretti; G Hintermann; R Hütter
Journal:  Plasmid       Date:  1985-09       Impact factor: 3.466

9.  The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages.

Authors:  F Boccard; T Smokvina; J L Pernodet; A Friedmann; M Guérineau
Journal:  EMBO J       Date:  1989-03       Impact factor: 11.598
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  9 in total

1.  Development of cloning vectors and transformation methods for Amycolatopsis.

Authors:  Gauri Dhingra; Rekha Kumari; Shashi Bala; Swati Majumdar; Shweta Malhotra; Poonam Sharma; Sukanya Lal; John Cullum; Rup Lal
Journal:  J Ind Microbiol Biotechnol       Date:  2003-04-08       Impact factor: 3.346

2.  Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors.

Authors:  C V Kumar; J J Coque; J F Martín
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

3.  Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.

Authors:  Wendy L Kelly; Craig A Townsend
Journal:  J Bacteriol       Date:  2005-01       Impact factor: 3.490

4.  The genus Amycolatopsis: Indigenous plasmids, cloning vectors and gene transfer systems.

Authors:  S Malhotra; R Lal
Journal:  Indian J Microbiol       Date:  2007-06-14       Impact factor: 2.461

5.  Metabolic pathway for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) formation in Nocardia corallina: inactivation of mutB by chromosomal integration of a kanamycin resistance gene.

Authors:  H F Valentin; D Dennis
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792

6.  Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina.

Authors:  Hong Gao; Buvani Murugesan; Janina Hoßbach; Stephanie K Evans; W Marshall Stark; Margaret C M Smith
Journal:  BMC Biotechnol       Date:  2019-06-04       Impact factor: 2.563

7.  Heterologous expression of the atypical tetracycline chelocardin reveals the full set of genes required for its biosynthesis.

Authors:  Tadeja Lukežič; Špela Pikl; Nestor Zaburannyi; Maja Remškar; Hrvoje Petković; Rolf Müller
Journal:  Microb Cell Fact       Date:  2020-12-19       Impact factor: 5.328

8.  Marker-Free Genome Engineering in Amycolatopsis Using the pSAM2 Site-Specific Recombination System.

Authors:  Luísa D F Santos; Laëtitia Caraty-Philippe; Emmanuelle Darbon; Jean-Luc Pernodet
Journal:  Microorganisms       Date:  2022-04-16

9.  The VanRS Homologous Two-Component System VnlRSAb of the Glycopeptide Producer Amycolatopsis balhimycina Activates Transcription of the vanHAXSc Genes in Streptomyces coelicolor, but not in A. balhimycina.

Authors:  Regina Kilian; Hans-Joerg Frasch; Andreas Kulik; Wolfgang Wohlleben; Evi Stegmann
Journal:  Microb Drug Resist       Date:  2016-07-15       Impact factor: 3.431
  9 in total

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