Literature DB >> 2999850

Isolation and characterization of an extrachromosomal element from Nocardia mediterranei.

P Moretti, G Hintermann, R Hütter.   

Abstract

Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization experiments showed that pMEA 100 is present in strain LBG A3136 in the free as well as in the integrated form whereas in strain ATCC 13685 only an integrated form was detected. Excision and reintegration in strain LBG A3136 seemed to be site specific. pMEA100 was found to be self-transmissible, eliciting the lethal zygosis phenotype, and is possibly involved in fertility in N. mediterranei.

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Year:  1985        PMID： 2999850     DOI： 10.1016/0147-619x(85)90072-1

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  18 in total

1.  Development of cloning vectors and transformation methods for Amycolatopsis.

Authors:  Gauri Dhingra; Rekha Kumari; Shashi Bala; Swati Majumdar; Shweta Malhotra; Poonam Sharma; Sukanya Lal; John Cullum; Rup Lal
Journal:  J Ind Microbiol Biotechnol       Date:  2003-04-08       Impact factor: 3.346

2.  Site-specific integration and excision of pMEA100 in Nocardia mediterranei.

Authors:  J Madon; P Moretti; R Hütter
Journal:  Mol Gen Genet       Date:  1987-09

3.  Construction of a hybrid plasmid capable of replication in Amycolatopsis mediterranei.

Authors:  R Lal; S Lal; E Grund; R Eichenlaub
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

4.  Conjugation of ϕBT1-derived integrative plasmid pDZL802 in Amycolatopsis mediterranei U32.

Authors:  Chen Li; Li Zhou; Ying Wang; Guoping Zhao; Xiaoming Ding
Journal:  Bioengineered       Date:  2017-01-03       Impact factor: 3.269

5.  Identification and functional analysis of the transfer region of plasmid pMEA300 of the methylotrophic actinomycete Amycolatopsis methanolica.

Authors:  J W Vrijbloed; N M van der Put; L Dijkhuizen
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

6.  Analysis of recombination occurring at SLP1 att sites.

Authors:  S C Lee; C A Omer; M A Brasch; S N Cohen
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

7.  A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration.

Authors:  J W Vrijbloed; J Madoń; L Dijkhuizen
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

8.  Transformation system for Amycolatopsis (Nocardia) mediterranei: direct transformation of mycelium with plasmid DNA.

Authors:  J Madoń; R Hütter
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

9.  Characterization of the genes and attachment sites for site-specific integration of plasmid pSE101 in Saccharopolyspora erythraea and Streptomyces lividans.

Authors:  D P Brown; K B Idler; D M Backer; S Donadio; L Katz
Journal:  Mol Gen Genet       Date:  1994-01

10.  Plasmid-borne resistance to arsenate, arsenite, cadmium, and chloramphenicol in a Rhodococcus species.

Authors:  E R Dabbs; G J Sole
Journal:  Mol Gen Genet       Date:  1988-01
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