Literature DB >> 7683186

Direct extraction and purification of rRNA for ecological studies.

M A Moran1, V L Torsvik, T Torsvik, R E Hodson.   

Abstract

Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.

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Year:  1993        PMID： 7683186      PMCID： PMC202208          DOI： 10.1128/aem.59.3.915-918.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

Review 1.  Molecular approaches for environmental monitoring of microorganisms.

Authors:  R M Atlas; G Sayler; R S Burlage; A K Bej
Journal:  Biotechniques       Date:  1992-05       Impact factor: 1.993

2.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

Authors:  R I Amann; B J Binder; R J Olson; S W Chisholm; R Devereux; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

3.  DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.

Authors:  William E Holben; Janet K Jansson; Barry K Chelm; James M Tiedje
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

4.  Rapid method for direct extraction of mRNA from seeded soils.

Authors:  Y L Tsai; M J Park; B H Olson
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

5.  Rapid method for direct extraction of DNA from soil and sediments.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

6.  Method for blot-hybridization analysis of mRNA molecules from Bacillus subtilis.

Authors:  N P Ambulos; E J Duvall; P S Lovett
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors:  S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

8.  Use of nuclepore filters for counting bacteria by fluorescence microscopy.

Authors:  J E Hobbie; R J Daley; S Jasper
Journal:  Appl Environ Microbiol       Date:  1977-05       Impact factor: 4.792

9.  Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.

Authors:  Y L Tsai; B H Olson
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

10.  Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

Authors:  D A Stahl; B Flesher; H R Mansfield; L Montgomery
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792
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  32 in total

1.  Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA.

Authors:  B M Duineveld; G A Kowalchuk; A Keijzer; J D van Elsas; J A van Veen
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

2.  Spatial changes in the bacterial community structure along a vertical oxygen gradient in flooded paddy soil cores.

Authors:  H Lüdemann; I Arth; W Liesack
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

3.  Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition.

Authors:  R I Griffiths; A S Whiteley; A G O'Donnell; M J Bailey
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

4.  Whole-cell versus total RNA extraction for analysis of microbial community structure with 16S rRNA-targeted oligonucleotide probes in salt marsh sediments.

Authors:  M E Frischer; J M Danforth; M A Newton Healy; F M Saunders
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792

5.  Simultaneous recovery of RNA and DNA from soils and sediments.

Authors:  R A Hurt; X Qiu; L Wu; Y Roh; A V Palumbo; J M Tiedje; J Zhou
Journal:  Appl Environ Microbiol       Date:  2001-10       Impact factor: 4.792

6.  The presence of humic substances and DNA in RNA extracts affects hybridization results.

Authors:  E W Alm; D Zheng; L Raskin
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

7.  Sulfate-reducing bacteria methylate mercury at variable rates in pure culture and in marine sediments.

Authors:  J K King; J E Kostka; M E Frischer; F M Saunders
Journal:  Appl Environ Microbiol       Date:  2000-06       Impact factor: 4.792

8.  Molecular phylogenetic and biogeochemical studies of sulfate-reducing bacteria in the rhizosphere of spartina alterniflora

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

9.  Molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16S rRNA, particulate methane monooxygenase, and methanol dehydrogenase

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

10.  Parallel characterization of anaerobic toluene- and ethylbenzene-degrading microbial consortia by PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization, and DNA microarray technology.

Authors:  Yoshikazu Koizumi; John J Kelly; Tatsunori Nakagawa; Hidetoshi Urakawa; Saïd El-Fantroussi; Saleh Al-Muzaini; Manabu Fukui; Yoshikuni Urushigawa; David A Stahl
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792
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