Literature DB >> 16535170

A Method for Purification of Clostridium perfringens Phospholipase C from Recombinant Bacillus subtilis Cells.

Y Hirata, J Minami, M Koyama, O Matsushita, S Katayama, F Jin, H Maeta, A Okabe.   

Abstract

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

Entities:  

Year:  1995        PMID： 16535170      PMCID： PMC1388606          DOI： 10.1128/aem.61.11.4114-4115.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  Cloning and sequencing of a phospholipase C gene of Clostridium perfringens.

Authors:  A Okabe; T Shimizu; H Hayashi
Journal:  Biochem Biophys Res Commun       Date:  1989-04-14       Impact factor: 3.575

2.  Phospholipase C mimics the differential effects of phorbol-12-myristate-13-acetate on the colony formation and cornification of cultured normal and transformed human keratinocytes.

Authors:  E K Parkinson
Journal:  Carcinogenesis       Date:  1987-06       Impact factor: 4.944

3.  Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.

Authors:  T Takahashi; T Sugahara; A Ohsaka
Journal:  Biochim Biophys Acta       Date:  1974-05-10

4.  Assay methods for alpha toxin from Clostridium perfringens: phospholipase C.

Authors:  C Jolivet-Reynaud; H Moreau; J E Alouf
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

Review 5.  Protein secretion in Bacillus species.

Authors:  M Simonen; I Palva
Journal:  Microbiol Rev       Date:  1993-03

Review 6.  Bacterial phospholipases C.

Authors:  R W Titball
Journal:  Microbiol Rev       Date:  1993-06

7.  Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.

Authors:  F Kawamura; R H Doi
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

8.  Regulation of Clostridium perfringens alpha-toxin-activated phospholipase C in rabbit erythrocyte membranes.

Authors:  J Sakurai; S Ochi; H Tanaka
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441

9.  Stable hyper-production of Escherichia coli beta-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a B. subtilis alpha-amylase secretion vector.

Authors:  K Nakamura; T Furusato; T Shiroza; K Yamane
Journal:  Biochem Biophys Res Commun       Date:  1985-04-30       Impact factor: 3.575

10.  Lethal effects and cardiovascular effects of purified alpha- and theta-toxins from Clostridium perfringens.

Authors:  D L Stevens; B E Troyer; D T Merrick; J E Mitten; R D Olson
Journal:  J Infect Dis       Date:  1988-02       Impact factor: 5.226
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