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Literature DB >> 6434524

Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases.

Abstract

A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development.

Entities: Chemical Species

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Year: 1984 PMID： 6434524 PMCID： PMC214740 DOI： 10.1128/jb.160.1.442-444.1984

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

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Authors: P J Piggot; J G Coote
Journal: Bacteriol Rev Date: 1976-12

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

Review 3. Genetics of bacterial sporulation.

Authors: J A Hoch
Journal: Adv Genet Date: 1976 Impact factor: 1.944

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Authors: H Uehara; K Yamane; B Maruo
Journal: J Bacteriol Date: 1979-08 Impact factor: 3.490

5. Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.

Authors: M L Stahl; E Ferrari
Journal: J Bacteriol Date: 1984-05 Impact factor: 3.490

6. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo.

Authors: S L Wong; C W Price; D S Goldfarb; R H Doi
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

7. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.

Authors: T J Gryczan; S Contente; D Dubnau
Journal: J Bacteriol Date: 1978-04 Impact factor: 3.490

8. Formation of competent Bacillus subtilis cells.

Authors: Y Sadaie; T Kada
Journal: J Bacteriol Date: 1983-02 Impact factor: 3.490

9. A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor.

Authors: D S Goldfarb; S L Wong; T Kudo; R H Doi
Journal: Mol Gen Genet Date: 1983

10. Bacillopeptidase F: two forms of a glycoprotein serine protease from Bacillus subtilis 168.

Authors: C A Roitsch; J H Hageman
Journal: J Bacteriol Date: 1983-07 Impact factor: 3.490

136 in total

1. Contribution of the cell wall component teichuronopeptide to pH homeostasis and alkaliphily in the alkaliphile Bacillus lentus C-125.

Authors: R Aono; M Ito; T Machida
Journal: J Bacteriol Date: 1999-11 Impact factor: 3.490

2. In vitro and in vivo analysis of transcription within the replication region of plasmid pIP501.

Authors: S Brantl; B Nuez; D Behnke
Journal: Mol Gen Genet Date: 1992-07

3. Molecular cloning, nucleotide sequence and expression of the structural gene for a thermostable alkaline protease from Bacillus sp. no. AH-101.

Authors: H Takami; T Kobayashi; R Aono; K Horikoshi
Journal: Appl Microbiol Biotechnol Date: 1992-10 Impact factor: 4.813

4. Extracellular production system of heterologous peptide driven by a secretory protease inhibitor of Streptomyces.

Authors: S Taguchi; M Maeno; H Momose
Journal: Appl Microbiol Biotechnol Date: 1992-03 Impact factor: 4.813

5. Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family.

Authors: S Tschauder; A J Driessen; R Freudl
Journal: Mol Gen Genet Date: 1992-10

6. Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE.

Authors: Mitsuo Ogura; Atsushi Matsuzawa; Hirofumi Yoshikawa; Teruo Tanaka
Journal: J Bacteriol Date: 2004-05 Impact factor: 3.490