Literature DB >> 16535057

Purification and Characterization of a Glucoamylase from Humicola grisea.

L Campos, C R Felix.   

Abstract

A thermostable extracellular glucoamylase from the thermophilic fungus Humicola grisea was purified to homogeneity. Its molecular mass and isoelectric point were 74 kDa and 8.4, respectively. The enzyme contained 5% carbohydrate, showed maximal activities at pH 6.0 and 60(deg)C, and was stable at 55(deg)C and pH 6.0 for 2 h. The K(infm) of soluble starch hydrolysis at 50(deg)C and pH 6.0 was 0.14 mg/ml. The purified enzyme was remarkably insensitive to glucose.

Entities:  

Year:  1995        PMID： 16535057      PMCID： PMC1388475          DOI： 10.1128/aem.61.6.2436-2438.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

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Authors:  J H PAZUR; T ANDO
Journal:  J Biol Chem       Date:  1959-08       Impact factor: 5.157

2.  Amylolytic activity of Humicola sp.

Authors:  A R de Oliveira; E de A Ximenes; C R Felix
Journal:  An Acad Bras Cienc       Date:  1991-12       Impact factor: 1.753

3.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

4.  Purification and properties of an extracellular conidial trehalase from Humicola grisea var. thermoidea.

Authors:  A L Zimmermann; H F Terenzi; J A Jorge
Journal:  Biochim Biophys Acta       Date:  1990-10-12

Review 5.  Designer enzyme and cell applications in industry and in environmental monitoring.

Authors:  A Wiseman
Journal:  J Chem Technol Biotechnol       Date:  1993       Impact factor: 3.174

6.  Structural similarities in glucoamylase by hydrophobic cluster analysis.

Authors:  P M Coutinho; P J Reilly
Journal:  Protein Eng       Date:  1994-06

7.  Structure-function relationships in the catalytic and starch binding domains of glucoamylase.

Authors:  P M Coutinho; P J Reilly
Journal:  Protein Eng       Date:  1994-03

8.  Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis.

Authors:  M R Sierks; C Ford; P J Reilly; B Svensson
Journal:  Protein Eng       Date:  1993-01

9.  Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis.

Authors:  M R Sierks; B Svensson
Journal:  Biochemistry       Date:  1993-02-02       Impact factor: 3.162

10.  Purification and properties of an extracellular glucoamylase from a diastatic strain of Saccharomyces cerevisiae.

Authors:  M J Kleinman; A E Wilkinson; I P Wright; I H Evans; E A Bevan
Journal:  Biochem J       Date:  1988-01-01       Impact factor: 3.857
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  5 in total

Review 1.  Thermophilic fungi: their physiology and enzymes.

Authors:  R Maheshwari; G Bharadwaj; M K Bhat
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2.  Dissolved oxygen as principal parameter for conidia production of biocontrol fungi Trichoderma viride in non-Newtonian wastewater.

Authors:  M Verma; Satinder K Brar; R D Tyagi; R Y Surampalli; J R Valéro
Journal:  J Ind Microbiol Biotechnol       Date:  2006-08-15       Impact factor: 3.346

3.  Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352.

Authors:  Vinayagam Ramesh; Vytla Ramachandra Murty
Journal:  Enzyme Res       Date:  2014-07-09

4.  Characterization of SdGA, a cold-adapted glucoamylase from Saccharophagus degradans.

Authors:  Natael M Wayllace; Nicolas Hedín; María V Busi; Diego F Gomez-Casati
Journal:  Biotechnol Rep (Amst)       Date:  2021-05-04

5.  Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis.

Authors:  Andrei Stecca Steindorff; Luana Assis Serra; Eduardo Fernandes Formighieri; Fabrícia Paula de Faria; Marcio José Poças-Fonseca; João Ricardo Moreira de Almeida
Journal:  Microbiol Spectr       Date:  2021-09-15
  5 in total

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