Literature DB >> 16511316

Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis.

Martin Ruppert1, Santosh Panjikar, Leif Barleben, Joachim Stöckigt.   

Abstract

Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 buffer and 11% PEG 4000 as precipitant. Crystals belong to space group I222 and diffract to 2.30 A, with unit-cell parameters a = 102.8, b = 127.3, c = 215.8 A.

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Year:  2006        PMID： 16511316      PMCID： PMC2197181          DOI： 10.1107/S174430910600457X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  16 in total

1.  Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli.

Authors:  K Walker; R Croteau
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

3.  In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium.

Authors:  Sylvestre Marillonnet; Anatoli Giritch; Mario Gils; Romy Kandzia; Victor Klimyuk; Yuri Gleba
Journal:  Proc Natl Acad Sci U S A       Date:  2004-04-21       Impact factor: 11.205

4.  Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in opium poppy Papaver somniferum.

Authors:  T Grothe; R Lenz; T M Kutchan
Journal:  J Biol Chem       Date:  2001-06-12       Impact factor: 5.157

5.  Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids.

Authors:  Irina Gerasimenko; Yuri Sheludko; Xueyan Ma; Joachim Stöckigt
Journal:  Eur J Biochem       Date:  2002-04

6.  Crystal structure of vinorine synthase, the first representative of the BAHD superfamily.

Authors:  Xueyan Ma; Juergen Koepke; Santosh Panjikar; Günter Fritzsch; Joachim Stöckigt
Journal:  J Biol Chem       Date:  2005-01-22       Impact factor: 5.157

7.  Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis.

Authors:  H Warzecha; I Gerasimenko; T M Kutchan; J Stöckigt
Journal:  Phytochemistry       Date:  2000-08       Impact factor: 4.072

8.  Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons.

Authors:  Leif Barleben; Xueyan Ma; Juergen Koepke; Guohong Peng; Hartmut Michel; Joachim Stöckigt
Journal:  Biochim Biophys Acta       Date:  2004-10-27

9.  Endogenous formation of morphine in human cells.

Authors:  Chotima Poeaknapo; Jürgen Schmidt; Matthias Brandsch; Birgit Dräger; Meinhart H Zenk
Journal:  Proc Natl Acad Sci U S A       Date:  2004-09-21       Impact factor: 11.205

10.  Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family.

Authors:  Xueyan Ma; Juergen Koepke; Günter Fritzsch; Ralf Diem; Toni M Kutchan; Hartmut Michel; Joachim Stöckigt
Journal:  Biochim Biophys Acta       Date:  2004-10-01
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  2 in total

1.  Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants.

Authors:  Cindy Rosenthal; Uwe Mueller; Santosh Panjikar; Lianli Sun; Martin Ruppert; Yu Zhao; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-30

2.  The nuclear genome of Rhazya stricta and the evolution of alkaloid diversity in a medically relevant clade of Apocynaceae.

Authors:  Jamal S M Sabir; Robert K Jansen; Dhivya Arasappan; Virginie Calderon; Emmanuel Noutahi; Chunfang Zheng; Seongjun Park; Meshaal J Sabir; Mohammed N Baeshen; Nahid H Hajrah; Mohammad A Khiyami; Nabih A Baeshen; Abdullah Y Obaid; Abdulrahman L Al-Malki; David Sankoff; Nadia El-Mabrouk; Tracey A Ruhlman
Journal:  Sci Rep       Date:  2016-09-22       Impact factor: 4.379
  2 in total

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