Literature DB >> 15680242

Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons.

Leif Barleben1, Xueyan Ma, Juergen Koepke, Guohong Peng, Hartmut Michel, Joachim Stöckigt.   

Abstract

Strictosidine beta-D-glucosidase, a plant enzyme initiating biosynthetic pathways to about 2000 monoterpenoid indole alkaloids with an extremely large number of various carbon skeletons, has been functionally expressed in Escherichia coli and purified to homogeneity in mg scale. Crystals suitable for X-ray analysis were found by robot-mediated screening. Using the hanging-drop technique, optimum conditions were 0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 and PEG 4000 (10%) as precipitant buffer. The crystals of strictosidine glucosidase belong to the space group P42(1)2 with unit cell dimensions of a=157.63, c=103.59 A and diffract X-rays to 2.48-A resolution.

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Year:  2004        PMID： 15680242     DOI： 10.1016/j.bbapap.2004.09.026

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis.

Authors:  Martin Ruppert; Santosh Panjikar; Leif Barleben; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-02-24

2.  Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants.

Authors:  Cindy Rosenthal; Uwe Mueller; Santosh Panjikar; Lianli Sun; Martin Ruppert; Yu Zhao; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-30

3.  Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.

Authors:  Martin Ruppert; Jörn Woll; Anatoli Giritch; Ezzat Genady; Xueyan Ma; Joachim Stöckigt
Journal:  Planta       Date:  2005-08-18       Impact factor: 4.116

4.  Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase.

Authors:  Sompong Sansenya; James R Ketudat Cairns; Rodjana Opassiri
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-02-25

5.  Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family.

Authors:  Leif Barleben; Santosh Panjikar; Martin Ruppert; Juergen Koepke; Joachim Stöckigt
Journal:  Plant Cell       Date:  2007-09-21       Impact factor: 11.277

Review 6.  Sarpagine and Related Alkaloids.

Authors:  Ojas A Namjoshi; James M Cook
Journal:  Alkaloids Chem Biol       Date:  2015-10-09

7.  Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase.

Authors:  Rodjana Opassiri; Busarakum Pomthong; Tassanee Onkoksoong; Takashi Akiyama; Asim Esen; James R Ketudat Cairns
Journal:  BMC Plant Biol       Date:  2006-12-29       Impact factor: 4.215

8.  Examining the transcriptional response of overexpressing anthranilate synthase in the hairy roots of an important medicinal plant Catharanthus roseus by RNA-seq.

Authors:  Jiayi Sun; Harish Manmathan; Cheng Sun; Christie A M Peebles
Journal:  BMC Plant Biol       Date:  2016-05-06       Impact factor: 4.215

9.  Catalytic properties, functional attributes and industrial applications of β-glucosidases.

Authors:  Gopal Singh; A K Verma; Vinod Kumar
Journal:  3 Biotech       Date:  2015-12-31       Impact factor: 2.406
  9 in total

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