Literature DB >> 15450856

Crystallization and preliminary X-ray crystallographic analysis of strictosidine synthase from Rauvolfia: the first member of a novel enzyme family.

Xueyan Ma1, Juergen Koepke, Günter Fritzsch, Ralf Diem, Toni M Kutchan, Hartmut Michel, Joachim Stöckigt.   

Abstract

Strictosidine synthase is a central enzyme involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids. Strictosidine synthase from Rauvolfia serpentina was heterologously expressed in Escherichia coli. Crystals of the purified recombinant enzyme have been obtained by the hanging-drop technique at 303 K with potassium sodium tartrate tetrahydrate as precipitant. The crystals belong to the space group R3 with cell dimensions of a=b=150.3 A and c=122.4 A. Under cryoconditions (120 K), the crystals diffract to about 2.95 A.

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Year:  2004        PMID： 15450856     DOI： 10.1016/j.bbapap.2004.06.013

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  11 in total

1.  Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis.

Authors:  Martin Ruppert; Santosh Panjikar; Leif Barleben; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-02-24

2.  The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.

Authors:  Xueyan Ma; Santosh Panjikar; Juergen Koepke; Elke Loris; Joachim Stöckigt
Journal:  Plant Cell       Date:  2006-03-10       Impact factor: 11.277

3.  Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants.

Authors:  Cindy Rosenthal; Uwe Mueller; Santosh Panjikar; Lianli Sun; Martin Ruppert; Yu Zhao; Joachim Stöckigt
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-30

4.  Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system.

Authors:  Martin Ruppert; Jörn Woll; Anatoli Giritch; Ezzat Genady; Xueyan Ma; Joachim Stöckigt
Journal:  Planta       Date:  2005-08-18       Impact factor: 4.116

5.  Molecular architecture of strictosidine glucosidase: the gateway to the biosynthesis of the monoterpenoid indole alkaloid family.

Authors:  Leif Barleben; Santosh Panjikar; Martin Ruppert; Juergen Koepke; Joachim Stöckigt
Journal:  Plant Cell       Date:  2007-09-21       Impact factor: 11.277

6.  Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in β-carboline skeleton biosynthesis.

Authors:  Hua Wang; Huaidong Zhang; Yanling Mi; Jianhua Ju; Qi Chen; Houjin Zhang
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-09-25       Impact factor: 1.056

7.  Strictosidine synthase: mechanism of a Pictet-Spengler catalyzing enzyme.

Authors:  Justin J Maresh; Lesley-Ann Giddings; Anne Friedrich; Elke A Loris; Santosh Panjikar; Bernhardt L Trout; Joachim Stöckigt; Baron Peters; Sarah E O'Connor
Journal:  J Am Chem Soc       Date:  2008-01-16       Impact factor: 15.419

Review 8.  Sarpagine and Related Alkaloids.

Authors:  Ojas A Namjoshi; James M Cook
Journal:  Alkaloids Chem Biol       Date:  2015-10-09

9.  LAP3, a novel plant protein required for pollen development, is essential for proper exine formation.

Authors:  Anna A Dobritsa; Shuh-Ichi Nishikawa; Daphne Preuss; Ewa Urbanczyk-Wochniak; Lloyd W Sumner; Adam Hammond; Ann L Carlson; Robert J Swanson
Journal:  Sex Plant Reprod       Date:  2009-06-11

10.  Asymmetric Synthesis of (R)-1-Alkyl-Substituted Tetrahydro-ß-carbolines Catalyzed by Strictosidine Synthases.

Authors:  Desiree Pressnitz; Eva-Maria Fischereder; Jakob Pletz; Christina Kofler; Lucas Hammerer; Katharina Hiebler; Horst Lechner; Nina Richter; Elisabeth Eger; Wolfgang Kroutil
Journal:  Angew Chem Int Ed Engl       Date:  2018-06-21       Impact factor: 15.336
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