Literature DB >> 16511177

Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.

Galina S Kachalova1, Rimma I Artyukh, Natalia V Lavrova, Elena M Ryazanova, Anna S Karyagina, Elena A Kubareva, Hans D Bartunik.   

Abstract

Crystals of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 A resolution at 100 K. The crystals belong to space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71 A. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume.

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Year:  2005        PMID： 16511177      PMCID： PMC1978117          DOI： 10.1107/S1744309105026709

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  22 in total

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5.  Cloning and analysis of the HaeIII and HaeII methyltransferase genes.

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6.  Structure of a binary complex of HhaI methyltransferase with S-adenosyl-L-methionine formed in the presence of a short non-specific DNA oligonucleotide.

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7.  Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA.

Authors:  A Dong; J A Yoder; X Zhang; L Zhou; T H Bestor; X Cheng
Journal:  Nucleic Acids Res       Date:  2001-01-15       Impact factor: 16.971

8.  Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase.

Authors:  D Labbé; H J Höltke; P C Lau
Journal:  Mol Gen Genet       Date:  1990-10

9.  Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method.

Authors:  E A Kubareva; J Walter; A S Karyagina; O V Vorob'eva; P C K Lau; T Trautner
Journal:  Biotechniques       Date:  2002-09       Impact factor: 1.993

10.  The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.

Authors:  Andrea Hermann; Sigrid Schmitt; Albert Jeltsch
Journal:  J Biol Chem       Date:  2003-06-06       Impact factor: 5.157
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