Literature DB >> 12794065

The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity.

Andrea Hermann1, Sigrid Schmitt, Albert Jeltsch.   

Abstract

The human Dnmt2 protein is one member of a protein family conserved from Schizosaccharomyces pombe and Drosophila melanogaster to Mus musculus and Homo sapiens. It contains all of the amino acid motifs characteristic for DNA-(Cytosine-C5) methyltransferases, and its structure is very similar to prokaryotic DNA methyltransferases. Nevertheless, so far all attempts to detect catalytic activity of this protein have failed. We show here by two independent assay systems that the purified Dnmt2 protein has weak DNA methyltransferase activity. Methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases.

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Year:  2003        PMID： 12794065     DOI： 10.1074/jbc.M305448200

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  61 in total

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2.  Evolution of gene sequence in response to chromosomal location.

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Authors:  Galina S Kachalova; Rimma I Artyukh; Natalia V Lavrova; Elena M Ryazanova; Anna S Karyagina; Elena A Kubareva; Hans D Bartunik
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-08-31

4.  In vitro tRNA methylation assay with the Entamoeba histolytica DNA and tRNA methyltransferase Dnmt2 (Ehmeth) enzyme.

Authors:  Ayala Tovy; Benjamin Hofmann; Mark Helm; Serge Ankri
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Review 5.  Solving the Dnmt2 enigma.

Authors:  Matthias Schaefer; Frank Lyko
Journal:  Chromosoma       Date:  2010-02       Impact factor: 4.316

6.  DNA methyltransferase expression differs with proliferation in childhood acute lymphoblastic leukemia.

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Journal:  Mol Biol Rep       Date:  2009-09-10       Impact factor: 2.316

7.  Epigenetic Basis for the Differentiation Potential of Mesenchymal and Embryonic Stem Cells.

Authors:  Philippe Collas; Agate Noer; Anita L Sørensen
Journal:  Transfus Med Hemother       Date:  2008-05-08       Impact factor: 3.747

8.  Evolution of dnmt-2 and mbd-2-like genes in the free-living nematodes Pristionchus pacificus, Caenorhabditis elegans and Caenorhabditis briggsae.

Authors:  Arturo Gutierrez; Ralf J Sommer
Journal:  Nucleic Acids Res       Date:  2004-12-02       Impact factor: 16.971

9.  Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism.

Authors:  Tomasz P Jurkowski; Madeleine Meusburger; Sameer Phalke; Mark Helm; Wolfgang Nellen; Gunter Reuter; Albert Jeltsch
Journal:  RNA       Date:  2008-06-20       Impact factor: 4.942

10.  Quantitative analysis of associations between DNA hypermethylation, hypomethylation, and DNMT RNA levels in ovarian tumors.

Authors:  M Ehrlich; C B Woods; M C Yu; L Dubeau; F Yang; M Campan; D J Weisenberger; Ti Long; B Youn; E S Fiala; P W Laird
Journal:  Oncogene       Date:  2006-04-27       Impact factor: 9.867
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