Literature DB >> 12238762

Determination of methylation site of DNA-methyltransferase NlaX by a hybrid method.

E A Kubareva1, J Walter, A S Karyagina, O V Vorob'eva, P C K Lau, T Trautner.   

Abstract

Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M.NlaX) from Neisseria lactamica was established to be the inner cytosine in the double-stranded pentanucleotide recognition sequence 5'-CCNGG-3' (where N = any nucleoside). 5-Methylcytosine (m5C) type modification by M-N1aX was confirmed by the use of oligonucleotide substrates that contain 5-fluoro-2'-deoxycytidine.

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Year:  2002        PMID： 12238762     DOI： 10.2144/02333st02

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  3 in total

1.  Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.

Authors:  Galina S Kachalova; Rimma I Artyukh; Natalia V Lavrova; Elena M Ryazanova; Anna S Karyagina; Elena A Kubareva; Hans D Bartunik
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-08-31

2.  Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system.

Authors:  Petr V Konarev; Galina S Kachalova; Alexandra Yu Ryazanova; Elena A Kubareva; Anna S Karyagina; Hans D Bartunik; Dmitri I Svergun
Journal:  PLoS One       Date:  2014-04-07       Impact factor: 3.240

3.  Kinetic Basis of the Bifunctionality of SsoII DNA Methyltransferase.

Authors:  Nadezhda A Timofeyeva; Alexandra Yu Ryazanova; Maxim V Norkin; Tatiana S Oretskaya; Olga S Fedorova; Elena A Kubareva
Journal:  Molecules       Date:  2018-05-16       Impact factor: 4.411
  3 in total

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