Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification of NADH-Nitrate Reductase by Affinity Chromatography.

Literature DB >> 16659410

Purification of NADH-Nitrate Reductase by Affinity Chromatography.

Abstract

ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.

Entities: Chemical Species

Year: 1975 PMID： 16659410 PMCID： PMC541940 DOI： 10.1104/pp.56.6.853

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

6 in total

1. Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.

Authors: S T Thompson; K H Cass; E Stellwagen
Journal: Proc Natl Acad Sci U S A Date: 1975-02 Impact factor: 11.205

2. Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.

Authors: L D Ryan; C S Vestling
Journal: Arch Biochem Biophys Date: 1974-01 Impact factor: 4.013

3. Properties of a nitrate reductase of Chlorella.

Authors: L P Solomonson; B Vennesland
Journal: Biochim Biophys Acta Date: 1972-06-23

4. Further purification and properties of Neurospora nitrate reductase.

Authors: R H Garrett; A Nason
Journal: J Biol Chem Date: 1969-06-10 Impact factor: 5.157

5. [Reciprocity of yeast-phosphofructokinase with dextran blue 2000].

Authors: G Kopperschläger; W Diezel; R Freyer; S Liebe; E Hofmann
Journal: Eur J Biochem Date: 1971-09-13

6. Activation of nitrate reductase by oxidation.

Authors: K Jetschmann; L P Solomonson; B Vennesland
Journal: Biochim Biophys Acta Date: 1972-08-17

6 in total

23 in total

1. Purification of Squash NADH:Nitrate Reductase by Zinc Chelate Affinity Chromatography.

Authors: M G Redinbaugh; W H Campbell
Journal: Plant Physiol Date: 1983-01 Impact factor: 8.340

2. Pyridine nucleotide specificity and other properties of purified nitrate reductase from Chlorella variegata.

Authors: C R Hipkin; B A Al-Bassam; P J Syrett
Journal: Planta Date: 1979-01 Impact factor: 4.116

3. Effect of ammonium and ferricyanide on nitrate utilization by Chlorella vulgaris.

Authors: E K Pistorius; E A Funkhouser; H Voss
Journal: Planta Date: 1978-01 Impact factor: 4.116

4. Nitrate reductase of Chlorella fusca: Partial purification, cytochrome content and presence of HCN after in vivo inactivation.

Authors: H S Gewitz; J Piefke; B Vennesland
Journal: Planta Date: 1978-01 Impact factor: 4.116

5. The role of nitrate and ammonium ions and light on the induction of nitrate reductase in maize leaves.

Authors: A Oaks; M Poulle; V J Goodfellow; L A Cass; H Deising
Journal: Plant Physiol Date: 1988-12 Impact factor: 8.340

6. Characterization of Nitrate Reductase from Corn Leaves (Zea mays cv W64A x W182E) : Two Molecular Forms of the Enzyme.

Authors: H Nakagawa; M Poulle; A Oaks
Journal: Plant Physiol Date: 1984-06 Impact factor: 8.340