Literature DB >> 24232684

Purification of acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyltransferase from Catharanthus roseus leaves.

W Fahn1, J Stöckigt.   

Abstract

The enzyme acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyltransferase which terminates vindoline biosynthesis has been isolated from Catharanthus roseus leaves, further characterized and purified to homogeneity by three step column chromatography and subsequent preparative isoelectric focusing. Kinetic properties concerning the enzyme reaction are discussed. Five multiple forms of the acetyl-transferase could be observed, each consisting of two subunits. This enzyme is now the best characterized of the enzymes involved in vindoline biosynthesis.

Entities:  

Year:  1990        PMID： 24232684     DOI： 10.1007/BF00270066

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  9 in total

1.  Developmental Regulation of Enzymes of Indole Alkaloid Biosynthesis in Catharanthus roseus.

Authors:  V De Luca; J A Fernandez; D Campbell; W G Kurz
Journal:  Plant Physiol       Date:  1988-02       Impact factor: 8.340

2.  Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.

Authors:  S T Thompson; K H Cass; E Stellwagen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Triazine-dye affinity; chromatography.

Authors:  T Atkinson; P M Hammond; R D Hartwell; P Hughes; M D Scawen; R F Sherwood; D A Small; C J Bruton; M J Harvey; C R Lowe
Journal:  Biochem Soc Trans       Date:  1981-08       Impact factor: 5.407

5.  Late enzymes of vindoline biosynthesis. Acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyl-transferase.

Authors:  W Fahn; H Gundlach; B Deus-Neumann; J Stöckigt
Journal:  Plant Cell Rep       Date:  1985-12       Impact factor: 4.570

6.  Late enzymes of vindoline biosynthesis : S-Adenosyl-L-methionine: 11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase and unspecific acetylesterase.

Authors:  W Fahn; E Laußermair; B Deus-Neumann; J Stöckigt
Journal:  Plant Cell Rep       Date:  1985-12       Impact factor: 4.570

7.  Subcellular Localization of Enzymes Involved in Indole Alkaloid Biosynthesis in Catharanthus roseus.

Authors:  V De Luca; A J Cutler
Journal:  Plant Physiol       Date:  1987-12       Impact factor: 8.340

8.  Mass Spectral Evidence of the Occurrence of Vindoline in Heterotrophic Cultures of Catharanthus roseus Cells.

Authors:  T Naaranlahti; S P Lapinjoki; A Huhtikangas; L Toivonen; U Kurtén; V Kauppinen; M Lounasmaa
Journal:  Planta Med       Date:  1989-04       Impact factor: 3.352

9.  Characterization of a novel N-methyltransferase (NMT) from Catharanthus roseus plants : Detection of NMT and other enzymes of the indole alkaloid biosynthetic pathway in different cell suspension culture systems.

Authors:  V Deluca; J Balsevich; R T Tyler; W G Kurz
Journal:  Plant Cell Rep       Date:  1987-12       Impact factor: 4.570
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.