Literature DB >> 16457587

Gene-specific characterization of human histone H2B by electron capture dissociation.

Nertila Siuti1, Michael J Roth, Craig A Mizzen, Neil L Kelleher, James J Pesavento.   

Abstract

The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.

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Year:  2006        PMID： 16457587     DOI： 10.1021/pr050268v

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  52 in total

Review 1.  The significance, development and progress of high-throughput combinatorial histone code analysis.

Authors:  Nicolas L Young; Peter A Dimaggio; Benjamin A Garcia
Journal:  Cell Mol Life Sci       Date:  2010-08-04       Impact factor: 9.261

2.  Label-Free Relative Quantitation of Isobaric and Isomeric Human Histone H2A and H2B Variants by Fourier Transform Ion Cyclotron Resonance Top-Down MS/MS.

Authors:  Xibei Dang; Amar Singh; Brian D Spetman; Krystal D Nolan; Jennifer S Isaacs; Jonathan H Dennis; Stephen Dalton; Alan G Marshall; Nicolas L Young
Journal:  J Proteome Res       Date:  2016-08-03       Impact factor: 4.466

3.  Unabridged Analysis of Human Histone H3 by Differential Top-Down Mass Spectrometry Reveals Hypermethylated Proteoforms from MMSET/NSD2 Overexpression.

Authors:  Yupeng Zheng; Luca Fornelli; Philip D Compton; Seema Sharma; Jesse Canterbury; Christopher Mullen; Vlad Zabrouskov; Ryan T Fellers; Paul M Thomas; Jonathan D Licht; Michael W Senko; Neil L Kelleher
Journal:  Mol Cell Proteomics       Date:  2015-08-13       Impact factor: 5.911

4.  Online matrix removal platform for coupling gel-based separations to whole protein electrospray ionization mass spectrometry.

Authors:  Ki Hun Kim; Philip D Compton; John C Tran; Neil L Kelleher
Journal:  J Proteome Res       Date:  2015-04-15       Impact factor: 4.466

5.  Liquid chromatography mass spectrometry profiling of histones.

Authors:  Xiaodan Su; Naduparambil K Jacob; Ravindra Amunugama; David M Lucas; Amy R Knapp; Chen Ren; Melanie E Davis; Guido Marcucci; Mark R Parthun; John C Byrd; Richard Fishel; Michael A Freitas
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2007-01-07       Impact factor: 3.205

6.  Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle.

Authors:  James J Pesavento; Hongbo Yang; Neil L Kelleher; Craig A Mizzen
Journal:  Mol Cell Biol       Date:  2007-10-29       Impact factor: 4.272

Review 7.  Decoding protein modifications using top-down mass spectrometry.

Authors:  Nertila Siuti; Neil L Kelleher
Journal:  Nat Methods       Date:  2007-10       Impact factor: 28.547

Review 8.  Accurate mass measurements in proteomics.

Authors:  Tao Liu; Mikhail E Belov; Navdeep Jaitly; Wei-Jun Qian; Richard D Smith
Journal:  Chem Rev       Date:  2007-07-25       Impact factor: 60.622

Review 9.  Mass spectrometry-based strategies for characterization of histones and their post-translational modifications.

Authors:  Xiaodan Su; Chen Ren; Michael A Freitas
Journal:  Expert Rev Proteomics       Date:  2007-04       Impact factor: 3.940

Review 10.  Top Down proteomics: facts and perspectives.

Authors:  Adam D Catherman; Owen S Skinner; Neil L Kelleher
Journal:  Biochem Biophys Res Commun       Date:  2014-02-17       Impact factor: 3.575
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