Statistical screening of medium components by Plackett-Burman design for lactic acid production by Lactobacillus sp. KCP01 using date juice.

Statistical screening of media components for production of lactic acid by Lactobacillus sp. KCP01 using date juice as a sugar source was carried out by Plackett-Burman design. Date juice at 5% sugar concentration when used alone showed 2.6 g/l of lactic acid production. Increase in lactic acid production (15.1 g/l) was observed with supplementation of salts and organic nitrogen sources of MRS medium and after optimization of pH and temperature using date juice as a C-source. Plackett-Burman design showed peptone, K2HPO4, sodium acetate and date juice as significant components influencing the lactic acid production.