Literature DB >> 163792

Escherichia coli enterotoxin: stimulation of adenylate cyclase in broken-cell preparations.

F Dorner, P Mayer.   

Abstract

The enterotoxin from cell-free filtrates of the enteropathogenic Escherichia coli strain P-263 was found to stimulate adenylate cyclase activity in broken-cell preparations from myocardial tissue. Particulate and detergent-solubilized fractions from cat heart were incubated with enterotoxin and assayed for adenylate cyclase activity. Adenylate cyclase activity was stimulated by enterotoxin; the extent of stimulation was proportional to the concentration of enterotoxin. The data demonstrate that stimulation of enterotoxin-sensitive adenylate cyclase in this system provides a sensitive in vitro assay, either as an accurate measure of enterotoxin concentration or as an assay for antitoxic titers in sera. A parallel comparison showed that stimulation of fluid production in rabbit intestinal loops by enterotoxin was less sensitive.

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Year:  1975        PMID： 163792      PMCID： PMC415081          DOI： 10.1128/iai.11.3.429-435.1975

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  22 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Hydrolysis of cyclic guanosine and adenosine 3',5'-monophosphates by rat and bovine tissues.

Authors:  J A Beavo; J G Hardman; E W Sutherland
Journal:  J Biol Chem       Date:  1970-11-10       Impact factor: 5.157

3.  Enterotoxigenic Escherichia coli isolated from patients with severe cholera-like disease.

Authors:  R B Sack; S L Gorbach; J G Banwell; B Jacobs; B D Chatterjee; R C Mitra
Journal:  J Infect Dis       Date:  1971-04       Impact factor: 5.226

4.  Comparative effects of enterotoxins from Escherichia coli and Vibrio cholerae on rabbit and swine small intestine.

Authors:  H W Moon; S C Whipp; A L Baetz
Journal:  Lab Invest       Date:  1971-08       Impact factor: 5.662

5.  Studies on enterotoxin from Escherichia coli associated with acute diarrhea in man.

Authors:  S Etkin; S L Gorbach
Journal:  J Lab Clin Med       Date:  1971-07

6.  The effect of cell-free fluids prepared from cultures of human and animal enteropathogenic strains of Escherichia coli on ligated intestinal segments of rabbits and pigs.

Authors:  H W Smith; C L Gyles
Journal:  J Med Microbiol       Date:  1970-08       Impact factor: 2.472

7.  The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin.

Authors:  H W Smith; C L Gyles
Journal:  J Med Microbiol       Date:  1970-08       Impact factor: 2.472

8.  The titration of cholera toxin and antitoxin in the rabbit ileal loop.

Authors:  G J Kasai; W Burrows
Journal:  J Infect Dis       Date:  1966-12       Impact factor: 5.226

9.  Cholera infection and toxin in the rabbit ileal loop.

Authors:  W Burrows; G M Musteikis
Journal:  J Infect Dis       Date:  1966-04       Impact factor: 5.226

10.  A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Authors:  A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1970-09       Impact factor: 11.205
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  9 in total

1.  Activation of adenylate cyclase by heat-labile Escherichia coli enterotoxin. Evidence for ADP-ribosyltransferase activity similar to that of choleragen.

Authors:  J Moss; S H Richardson
Journal:  J Clin Invest       Date:  1978-08       Impact factor: 14.808

2.  Stimulation of cyclic AMP secretion in Vero cells by enterotoxins of Escherichia coli and Vibrio cholerae.

Authors:  S Stavric; J I Speirs; J Konowalchuk; D Jeffrey
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

Review 3.  Pathophysiological effects of Vibrio cholerae and enterotoxigenic Escherichia coli and their exotoxins on eucaryotic cells.

Authors:  K L Richards; S D Douglas
Journal:  Microbiol Rev       Date:  1978-09

4.  Mechanism of action of choleragen and E. coli heat-labile enterotoxin: activation of adenylate cyclase by ADP-ribosylation.

Authors:  J Moss; M Vaughan
Journal:  Mol Cell Biochem       Date:  1981-07-07       Impact factor: 3.396

5.  Effect of lymphocytosis-promoting factor from Bordetella pertussis on cerebellar cyclic GMP levels.

Authors:  P Askelöf; P Gillenius
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

6.  Assay of Escherichia coli heat-labile enterotoxin with vero cells.

Authors:  J I Speirs; S Stavric; J Konowalchuk
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

7.  The effect of adsorbant and anti-inflammatory drugs on secretion in ligated segments of pig intestine infected with Escherichia coli.

Authors:  C L Gyles; M Zigler
Journal:  Can J Comp Med       Date:  1978-07

8.  Assay of Escherichia coli enterotoxins by in vivo perfusion in the rat jejunum.

Authors:  F A Klipstein; C S Lee; R F Engert
Journal:  Infect Immun       Date:  1976-10       Impact factor: 3.441

9.  Functional significance of histologic alterations induced by Escherichia coli pig-specific, mouse-negative, heat-stable enterotoxin (STb).

Authors:  S C Whipp; E Kokue; R W Morgan; R Rose; H W Moon
Journal:  Vet Res Commun       Date:  1987       Impact factor: 2.459
  9 in total

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