Literature DB >> 4318781

A protein binding assay for adenosine 3':5'-cyclic monophosphate.

A G Gilman.   

Abstract

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

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Year:  1970        PMID： 4318781      PMCID： PMC283204          DOI： 10.1073/pnas.67.1.305

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Determination of guanosine 3',5'-monophosphate in tissues and of guanyl cyclase in rat intestine.

Authors:  E Ishikawa; S Ishikawa; J W Davis; E W Sutherland
Journal:  J Biol Chem       Date:  1969-12-10       Impact factor: 5.157

2.  The assay of adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in biological materials by enzymatic radioisotopic displacement.

Authors:  G Brooker; L J Thomas; M M Appleman
Journal:  Biochemistry       Date:  1968-12       Impact factor: 3.162

3.  Enzymic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine.

Authors:  N D Goldberg; J Larner; H Sasko; A G O'Toole
Journal:  Anal Biochem       Date:  1969-04-04       Impact factor: 3.365

4.  Cyclic guanosine 3',5'-monophosphate in mammalian tissues and urine.

Authors:  N D Goldberg; S B Dietz; A G O'Toole
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

5.  The influence of chemical agents on the accumulation of adenosine 3',5'-Phosphate in slices of rabbit cerebellum.

Authors:  S Kakiuchi; T W Rall
Journal:  Mol Pharmacol       Date:  1968-07       Impact factor: 4.436

6.  An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle.

Authors:  D A Walsh; J P Perkins; E G Krebs
Journal:  J Biol Chem       Date:  1968-07-10       Impact factor: 5.157

7.  Factors affecting the activity of muscle glycogen synthetase. 3. The reaction with adenosine triphosphate Mg++, and cyclic 3'5'-adenosine monophosphate.

Authors:  M M Appleman; L Birnbaumer; H N Torres
Journal:  Arch Biochem Biophys       Date:  1966-09-26       Impact factor: 4.013

8.  Adenosine 3',5'-monophosphate in biological materials. II. The measurement of adenosine 3',5'-monophosphate in tissues and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine.

Authors:  R W Butcher; R J Ho; H C Meng; E W Sutherland
Journal:  J Biol Chem       Date:  1965-11       Impact factor: 5.157

9.  Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate.

Authors:  T A Langan
Journal:  Science       Date:  1968-11-01       Impact factor: 47.728

10.  Determination of 3',5'-adenosine monophosphate with a method based on a radioactive phosphate exchange reaction.

Authors:  G D Aurbach; B A Houston
Journal:  J Biol Chem       Date:  1968-11-25       Impact factor: 5.157
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  771 in total

1.  Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte.

Authors:  M H Wisher; W H Evans
Journal:  Biochem J       Date:  1975-02       Impact factor: 3.857

2.  Maturation of neuroblastoma cells in the presence of dimethylsulfoxide.

Authors:  Y Kimhi; C Palfrey; I Spector; Y Barak; U Z Littauer
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

3.  Effect of prolonged ethanol ingestion on hepatic lipogenesis and related enzyme activities.

Authors:  M J Savolainen; J K Hiltunen; I E Hassinen
Journal:  Biochem J       Date:  1977-04-15       Impact factor: 3.857

4.  Effects on body temperature of rats produced by prostaglandins, endotoxin, lipid A and antipyretics.

Authors:  W Feldberg; P N Saxena
Journal:  J Physiol       Date:  1975-02       Impact factor: 5.182

5.  Head movement during low-frequency vibration.

Authors:  J Sandover; R W Soames
Journal:  J Physiol       Date:  1975-05       Impact factor: 5.182

6.  Modulation of FSH receptor phosphorylation correlates with hormone-induced coupling to the adenylate cyclase system.

Authors:  N Selvaraj; A Amsterdam
Journal:  Endocrine       Date:  1997-04       Impact factor: 3.633

7.  Co-ordinated changes in the cyclic AMP signalling system and the phosphorylation of two nuclear proteins of Mr 130,000 and 110,000 during proliferative stimulation of the rat parotid gland by isoprenaline. Possible identity of the two proteins with pp135 and nucleolin.

Authors:  J Hoffmann; G Schwoch
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857

8.  Desensitization of alpha 2-adrenergic receptors in NG 108 15 cells by (-)-adrenaline and phorbol 12-myristate 13-acetate.

Authors:  A Convents; J P De Backer; C André; G Vauquelin
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

9.  Failure to Detect Cyclic 3', 5'-Adenosine Monophosphate in Healthy and Crown Gall Tumorous Tissues of Vicia faba.

Authors:  R M Niles; M S Mount
Journal:  Plant Physiol       Date:  1974-09       Impact factor: 8.340

10.  Formation of adenosine 5'-phosphorofluoridate and the assay of adenyl cyclase in barley seeds.

Authors:  R Alvarez
Journal:  Plant Physiol       Date:  1974-02       Impact factor: 8.340
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