Literature DB >> 16350952

The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information.

Tatsuya Sawasaki1, Mudeppa D Gouda, Takayasu Kawasaki, Takafumi Tsuboi, Yuzuru Tozawa, Kazuyuki Takai, Yaeta Endo.   

Abstract

This chapter contains protocols for high-throughput protein production based on the cell-free system prepared from eukaryote wheat embryos.

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Year:  2005        PMID： 16350952     DOI： 10.1007/978-1-59259-948-6_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  NMR-based characterization of a refolding intermediate of beta2-microglobulin labeled using a wheat germ cell-free system.

Authors:  Atsushi Kameda; Eugene-Hayato Morita; Kazumasa Sakurai; Hironobu Naiki; Yuji Goto
Journal:  Protein Sci       Date:  2009-08       Impact factor: 6.725

2.  Expression of functional Plasmodium falciparum enzymes using a wheat germ cell-free system.

Authors:  Devaraja G Mudeppa; Pradipsinh K Rathod
Journal:  Eukaryot Cell       Date:  2013-10-11

3.  Practical cell-free protein synthesis system using purified wheat embryos.

Authors:  Kazuyuki Takai; Tatsuya Sawasaki; Yaeta Endo
Journal:  Nat Protoc       Date:  2010-01-21       Impact factor: 13.491

4.  Overview of cell-free protein synthesis: historic landmarks, commercial systems, and expanding applications.

Authors:  Shaorong Chong
Journal:  Curr Protoc Mol Biol       Date:  2014-10-01

5.  Sterile protection against Plasmodium knowlesi in rhesus monkeys from a malaria vaccine: comparison of heterologous prime boost strategies.

Authors:  George Jiang; Meng Shi; Solomon Conteh; Nancy Richie; Glenna Banania; Harini Geneshan; Anais Valencia; Priti Singh; Joao Aguiar; Keith Limbach; Kurt I Kamrud; Jonathan Rayner; Jonathan Smith; Joseph T Bruder; C Richter King; Takafumi Tsuboi; Satoru Takeo; Yaeta Endo; Denise L Doolan; Thomas L Richie; Walter R Weiss
Journal:  PLoS One       Date:  2009-08-10       Impact factor: 3.240

6.  Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.

Authors:  Kazuhiro Matsuoka; Hiroaki Komori; Masato Nose; Yaeta Endo; Tatsuya Sawasaki
Journal:  J Proteome Res       Date:  2010-08-06       Impact factor: 4.466

7.  Paraquat toxicity induced by voltage-dependent anion channel 1 acts as an NADH-dependent oxidoreductase.

Authors:  Hiroki Shimada; Kei-Ichi Hirai; Eriko Simamura; Toshihisa Hatta; Hiroki Iwakiri; Keiji Mizuki; Taizo Hatta; Tatsuya Sawasaki; Satoko Matsunaga; Yaeta Endo; Shigeomi Shimizu
Journal:  J Biol Chem       Date:  2009-08-28       Impact factor: 5.157

8.  Identification of RFPL3 protein as a novel E3 ubiquitin ligase modulating the integration activity of human immunodeficiency virus, type 1 preintegration complex using a microtiter plate-based assay.

Authors:  Beng Hui Tan; Yasutsugu Suzuki; Hirotaka Takahashi; Pamela Ho Rui Ying; Chikako Takahashi; Qi'En Han; Wei Xin Chin; Sheng-Hao Chao; Tatsuya Sawasaki; Naoki Yamamoto; Youichi Suzuki
Journal:  J Biol Chem       Date:  2014-08-08       Impact factor: 5.157

9.  Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast.

Authors:  Takuya Maeda; Tomoya Saito; Omar S Harb; David S Roos; Satoru Takeo; Hiroko Suzuki; Takafumi Tsuboi; Tsutomu Takeuchi; Takashi Asai
Journal:  Parasitol Int       Date:  2008-10-31       Impact factor: 2.230

10.  A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis.

Authors:  Hirotaka Takahashi; Akira Nozawa; Motoaki Seki; Kazuo Shinozaki; Yaeta Endo; Tatsuya Sawasaki
Journal:  BMC Plant Biol       Date:  2009-04-06       Impact factor: 4.215
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