Literature DB >> 16350949

High-throughput cloning for proteomics research.

Sharon A Doyle1.   

Abstract

Ligation-independent cloning (LIC) is a simple, rapid, and efficient method for high-throughput cloning. In this system, linear plasmid vector and insert DNA are treated to generate complementary single-stranded overhangs that anneal during a short incubation. The LIC system is adaptable for use with any vector following an alteration of the vector sequence. This chapter describes the creation of an LIC-compatible vector, with tips on how to make any vector LIC-enabled. It also includes a protocol for generating high-quality linearized vector template for the LIC reaction. Lastly, a step-by-step protocol of the LIC reaction is outlined, with useful tips and tricks for optimization and screening.

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Year:  2005        PMID： 16350949     DOI： 10.1007/978-1-59259-948-6_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  20 in total

1.  Enzyme free cloning for high throughput gene cloning and expression.

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Authors:  Seychelle M Vos; Nichole K Stewart; Martha G Oakley; James M Berger
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3.  A monoclinic crystal form of casein kinase 1 δ.

Authors:  Nicholas A Zeringo; Lea Murphy; Eric A McCloskey; Loren Rohal; John J Bellizzi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2013-09-28

4.  A versatile set of ligation-independent cloning vectors for functional studies in plants.

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Journal:  Plant Physiol       Date:  2011-05-11       Impact factor: 8.340

5.  Distinct Mechanism Evolved for Mycobacterial RNA Polymerase and Topoisomerase I Protein-Protein Interaction.

Authors:  Srikanth Banda; Nan Cao; Yuk-Ching Tse-Dinh
Journal:  J Mol Biol       Date:  2017-08-24       Impact factor: 5.469

6.  Biochemical and EPR-spectroscopic investigation into heterologously expressed vinyl chloride reductive dehalogenase (VcrA) from Dehalococcoides mccartyi strain VS.

Authors:  Anutthaman Parthasarathy; Troy A Stich; Svenja T Lohner; Ann Lesnefsky; R David Britt; Alfred M Spormann
Journal:  J Am Chem Soc       Date:  2015-03-04       Impact factor: 15.419

7.  Two neonatal diabetes mutations on transmembrane helix 15 of SUR1 increase affinity for ATP and ADP at nucleotide binding domain 2.

Authors:  David Ortiz; Peter Voyvodic; Lindsay Gossack; Ulrich Quast; Joseph Bryan
Journal:  J Biol Chem       Date:  2012-03-27       Impact factor: 5.157

8.  Flexi vector cloning.

Authors:  Paul G Blommel; Peter A Martin; Kory D Seder; Russell L Wrobel; Brian G Fox
Journal:  Methods Mol Biol       Date:  2009

9.  Asp-to-Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli.

Authors:  Bokun Cheng; Thirunavukkarasu Annamalai; Elena Sorokin; Maria Abrenica; Sandra Aedo; Yuk-Ching Tse-Dinh
Journal:  J Mol Biol       Date:  2008-11-05       Impact factor: 5.469

10.  Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol.

Authors:  Thirunavukkarasu Annamalai; Neil Dani; Bokun Cheng; Yuk-Ching Tse-Dinh
Journal:  BMC Biochem       Date:  2009-06-11       Impact factor: 4.059
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