Literature DB >> 16348166

Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri.

T L Talarico1, W J Dobrogosz.   

Abstract

A coenzyme B(12)-dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. K(m) values for substrates and coenzyme B(12) were in the millimolar and the submicromolar range, respectively.

Entities:  

Year:  1990        PMID: 16348166      PMCID: PMC184372          DOI: 10.1128/aem.56.4.1195-1197.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  11 in total

1.  A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus.

Authors:  K L SMILEY; M SOBOLOV
Journal:  Arch Biochem Biophys       Date:  1962-06       Impact factor: 4.013

2.  An antigenic analysis of Lactobacillus acidophilus.

Authors:  C EFTHYMIOU; P A HANSEN
Journal:  J Infect Dis       Date:  1962 May-Jun       Impact factor: 5.226

3.  Metabolism of glycerol by an acrolein-forming lactobacillus.

Authors:  M SOBOLOV; K L SMILEY
Journal:  J Bacteriol       Date:  1960-02       Impact factor: 3.490

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Study of the mechanism of action of adenosylcobalamindependent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation kinetics of glycerol dehydratase complexes with adenosylobalamin and its analogs.

Authors:  A A Poznanskaya; M I Yakusheva; V A Yakovlev
Journal:  Biochim Biophys Acta       Date:  1977-09-15

6.  Coenzyme B12-dependent diol dehydrase: purification, subunit heterogeneity, and reversible association.

Authors:  A A Poznanskaja; K Tanizawa; K Soda; T Toraya; S Fukui
Journal:  Arch Biochem Biophys       Date:  1979-05       Impact factor: 4.013

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri.

Authors:  T L Talarico; I A Casas; T C Chung; W J Dobrogosz
Journal:  Antimicrob Agents Chemother       Date:  1988-12       Impact factor: 5.191

9.  Immunochemical evidence for the difference between coenzyme-B12-dependent diol dehydratase and glycerol dehydratase.

Authors:  T Toraya; S Fukui
Journal:  Eur J Biochem       Date:  1977-06-01

10.  Chemical characterization of an antimicrobial substance produced by Lactobacillus reuteri.

Authors:  T L Talarico; W J Dobrogosz
Journal:  Antimicrob Agents Chemother       Date:  1989-05       Impact factor: 5.191

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  23 in total

1.  Analysis of antimicrobial and immunomodulatory substances produced by heterofermentative Lactobacillus reuteri.

Authors:  Gabriela Greifová; Hyacinta Májeková; Gabriel Greif; Patrik Body; Maria Greifová; Martina Dubničková
Journal:  Folia Microbiol (Praha)       Date:  2017-04-11       Impact factor: 2.099

2.  Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri: Purification of 1,3-Propanediol:NAD Oxidoreductase.

Authors:  T L Talarico; L T Axelsson; J Novotny; M Fiuzat; W J Dobrogosz
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

3.  Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.

Authors:  M Seyfried; R Daniel; G Gottschalk
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

4.  1,3-Propanediol:NAD+ oxidoreductases of Lactobacillus brevis and Lactobacillus buchneri.

Authors:  M Veiga-da-Cunha; M A Foster
Journal:  Appl Environ Microbiol       Date:  1992-06       Impact factor: 4.792

5.  Production of 1,3-propanediol by Lactobacillus diolivorans from agro-industrial residues and cactus cladode acid hydrolyzate.

Authors:  Juliana Silva de Santana; Juliana Luna da Silva; Emmanuel Damilano Dutra; Rômulo Simões Cezar Menezes; Rafael Barros de Souza; Irapuan Oliveira Pinheiro
Journal:  Appl Biochem Biotechnol       Date:  2021-01-28       Impact factor: 2.926

6.  A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to L-alanine-mediated germination.

Authors:  R Moriyama; A Hattori; S Miyata; S Kudoh; S Makino
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

Review 7.  Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.

Authors:  Peng Zhao; Pingfang Tian
Journal:  World J Microbiol Biotechnol       Date:  2021-06-15       Impact factor: 3.312

8.  Sugar-glycerol cofermentations in lactobacilli: the fate of lactate.

Authors:  M Veiga da Cunha; M A Foster
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

9.  Lactobacillus reuteri DSM 20016 produces cobalamin-dependent diol dehydratase in metabolosomes and metabolizes 1,2-propanediol by disproportionation.

Authors:  Dinesh Diraviam Sriramulu; Mingzhi Liang; Diana Hernandez-Romero; Evelyne Raux-Deery; Heinrich Lünsdorf; Joshua B Parsons; Martin J Warren; Michael B Prentice
Journal:  J Bacteriol       Date:  2008-05-09       Impact factor: 3.490

10.  Next-Generation Probiotics Targeting Clostridium difficile through Precursor-Directed Antimicrobial Biosynthesis.

Authors:  Jennifer K Spinler; Jennifer Auchtung; Aaron Brown; Prapaporn Boonma; Numan Oezguen; Caná L Ross; Ruth Ann Luna; Jessica Runge; James Versalovic; Alex Peniche; Sara M Dann; Robert A Britton; Anthony Haag; Tor C Savidge
Journal:  Infect Immun       Date:  2017-09-20       Impact factor: 3.441

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