Literature DB >> 8824629

Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii.

M Seyfried1, R Daniel, G Gottschalk.   

Abstract

The genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii were cloned and overexpressed in Escherichia coli. The B12-free enzyme was purified to homogeneity. It consists of three types of subunits whose N-terminal sequences are in accordance with those deduced from the open reading frames dhaB, dhaC, and dhaE, coding for subunits of 60,433 (alpha), 21,487 (beta), and 16,121 (gamma) Da, respectively. The enzyme complex has the composition alpha2beta2gamma2. Amino acid alignments with the subunits of the recently sequenced diol dehydratase of Klebsiella oxytoca (T. Tobimatsu, T. Hara, M. Sakaguchi, Y. Kishimoto, Y. Wada, M. Isoda, T. Sakai, and T. Toraya, J. Biol. Chem. 270:7142-7148, 1995) revealed identities between 51.8 and 70.9%.

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Year:  1996        PMID： 8824629      PMCID： PMC178423          DOI： 10.1128/jb.178.19.5793-5796.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum. Homologies with other cobalamin-dependent enzymes.

Authors:  E N Marsh; D E Holloway
Journal:  FEBS Lett       Date:  1992-09-28       Impact factor: 4.124

2.  A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus.

Authors:  K L SMILEY; M SOBOLOV
Journal:  Arch Biochem Biophys       Date:  1962-06       Impact factor: 4.013

3.  Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri.

Authors:  T L Talarico; W J Dobrogosz
Journal:  Appl Environ Microbiol       Date:  1990-04       Impact factor: 4.792

4.  Resolution of the coenzyme B-12-dependent dehydratases of Klebsiella sp. and Citrobacter freundii.

Authors:  R G Forage; M A Foster
Journal:  Biochim Biophys Acta       Date:  1979-08-15

5.  Coenzyme B12-dependent diol dehydrase: purification, subunit heterogeneity, and reversible association.

Authors:  A A Poznanskaja; K Tanizawa; K Soda; T Toraya; S Fukui
Journal:  Arch Biochem Biophys       Date:  1979-05       Impact factor: 4.013

6.  Purification and properties of glycerol dehydrase.

Authors:  Z Schneider; E G Larsen; G Jacobson; B C Johnson; J Pawelkiewicz
Journal:  J Biol Chem       Date:  1970-07-10       Impact factor: 5.157

7.  The properties of glycerol dehydratase isolated from Aerobacter aerogenes, and the properties of the apoenzyme subunits.

Authors:  Z Schneider; J Pawelkiewicz
Journal:  Acta Biochim Pol       Date:  1966       Impact factor: 2.149

8.  Growth temperature-dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon.

Authors:  R Daniel; G Gottschalk
Journal:  FEMS Microbiol Lett       Date:  1992-12-15       Impact factor: 2.742

9.  A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

10.  How a protein binds B12: A 3.0 A X-ray structure of B12-binding domains of methionine synthase.

Authors:  C L Drennan; S Huang; J T Drummond; R G Matthews; M L Ludwig
Journal:  Science       Date:  1994-12-09       Impact factor: 47.728
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  10 in total

1.  Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli.

Authors:  Anja Knietsch; Tanja Waschkowitz; Susanne Bowien; Anke Henne; Rolf Daniel
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

2.  Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures.

Authors:  S Saint-Amans; L Girbal; J Andrade; K Ahrens; P Soucaille
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

3.  The propanediol utilization (pdu) operon of Salmonella enterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B(12)-dependent 1, 2-propanediol degradation.

Authors:  T A Bobik; G D Havemann; R J Busch; D S Williams; H C Aldrich
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

4.  Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii.

Authors:  Xianghui Qi; Wenying Deng; Fei Wang; Qi Guo; Huayou Chen; Liang Wang; Xiang He; Ribo Huang
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695

5.  Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum.

Authors:  Céline Raynaud; Patricia Sarçabal; Isabelle Meynial-Salles; Christian Croux; Philippe Soucaille
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-18       Impact factor: 11.205

6.  Inactivation of dhaD and dhaK abolishes by-product accumulation during 1,3-propanediol production in Klebsiella pneumoniae.

Authors:  Yu-Tze Horng; Kai-Chih Chang; Ta-Chung Chou; Chung-Jen Yu; Chih-Ching Chien; Yu-Hong Wei; Po-Chi Soo
Journal:  J Ind Microbiol Biotechnol       Date:  2010-04-09       Impact factor: 3.346

7.  Propanediol utilization genes (pdu) of Salmonella typhimurium: three genes for the propanediol dehydratase.

Authors:  T A Bobik; Y Xu; R M Jeter; K E Otto; J R Roth
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

8.  The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins.

Authors:  E Kofoid; C Rappleye; I Stojiljkovic; J Roth
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

9.  Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures.

Authors:  Anja Knietsch; Susanne Bowien; Gregg Whited; Gerhard Gottschalk; Rolf Daniel
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

Review 10.  Key enzymes catalyzing glycerol to 1,3-propanediol.

Authors:  Wei Jiang; Shizhen Wang; Yuanpeng Wang; Baishan Fang
Journal:  Biotechnol Biofuels       Date:  2016-03-10       Impact factor: 6.040
  10 in total

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