Literature DB >> 16347783

Improved Assay for Rhodanese in Thiobacillus spp.

D R Singleton1, D W Smith.   

Abstract

Rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Conventional rhodanese assays colorimetrically measure the formation of one or the other of the products. These assays suffer from the fact that there is significant nonbiological formation of these products in addition to the enzymatically catalyzed reaction. In the present report, we describe a modified procedure for assaying rhodanese in which a separate boiled control was prepared for each assay trial. The boiled control corrected for the nonbiological contributions to product formation.

Entities:  

Year:  1988        PMID： 16347783      PMCID： PMC204388          DOI： 10.1128/aem.54.11.2866-2867.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  MECHANISM OF RHODANESE CATALYSIS OF THIOSULFATE-LIPOATE OXIDATION-REDUCTION.

Authors:  M VILLAREJO; J WESTLEY
Journal:  J Biol Chem       Date:  1963-12       Impact factor: 5.157

2.  PATH OF SULFUR IN SULFIDE AND THIOSULFATE OXIDATION BY THIOBACILLI.

Authors:  J LONDON; S C RITTENBERG
Journal:  Proc Natl Acad Sci U S A       Date:  1964-11       Impact factor: 11.205

3.  Nitrogenase of Klebsiella pneumoniae. Rhodanese-catalysed restoration of activity of the inactive 2Fe species of the Fe protein.

Authors:  S Pagani; M Eldridge; R R Eady
Journal:  Biochem J       Date:  1987-06-01       Impact factor: 3.857

4.  Passage of a membrane protein through the walls of toluene-treated Bacillus megaterium cells.

Authors:  D P Fan; G L Gardner-Eckstrom
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

Review 5.  Mechanisms of inorganic oxidation and energy coupling.

Authors:  I Suzuki
Journal:  Annu Rev Microbiol       Date:  1974       Impact factor: 15.500

6.  Insertion of sulfide into ferredoxins catalyzed by rhodanese.

Authors:  F Bonomi; S Pagani; P Cerletti
Journal:  FEBS Lett       Date:  1977-12-01       Impact factor: 4.124

7.  Thiosulphate metabolism and rhodanese in Chromatium sp. strain D.

Authors:  A J Smith; J Lascelles
Journal:  J Gen Microbiol       Date:  1966-03

8.  Some properties of the rhodanese system of Thiobacillus denitrificans.

Authors:  T J Bowen; P J Butler; F C Happold
Journal:  Biochem J       Date:  1965-12       Impact factor: 3.857

9.  Release of rhodanese from Pseudomonas aeruginosa by cold shock and its localization within the cell.

Authors:  R W Ryan; M P Gourlie; R C Tilton
Journal:  Can J Microbiol       Date:  1979-03       Impact factor: 2.419

10.  Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier.

Authors:  S Silver; L Wendt
Journal:  J Bacteriol       Date:  1967-02       Impact factor: 3.490
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  4 in total

1.  A dual role of the transcriptional regulator TstR provides insights into cyanide detoxification in Lactobacillus brevis.

Authors:  Fernando A Pagliai; Caitlin C Murdoch; Sara M Brown; Claudio F Gonzalez; Graciela L Lorca
Journal:  Mol Microbiol       Date:  2014-04-14       Impact factor: 3.501

2.  Enzymatic and genetic characterization of carbon and energy metabolisms by deep-sea hydrothermal chemolithoautotrophic isolates of Epsilonproteobacteria.

Authors:  Ken Takai; Barbara J Campbell; S Craig Cary; Masae Suzuki; Hanako Oida; Takuro Nunoura; Hisako Hirayama; Satoshi Nakagawa; Yohey Suzuki; Fumio Inagaki; Koki Horikoshi
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

3.  An exported rhodanese-like protein is induced during growth of Acidithiobacillus ferrooxidans in metal sulfides and different sulfur compounds.

Authors:  Pablo Ramírez; Héctor Toledo; Nicolas Guiliani; Carlos A Jerez
Journal:  Appl Environ Microbiol       Date:  2002-04       Impact factor: 4.792

4.  Complete genome sequence of Spirochaeta smaragdinae type strain (SEBR 4228).

Authors:  Konstantinos Mavromatis; Montri Yasawong; Olga Chertkov; Alla Lapidus; Susan Lucas; Matt Nolan; Tijana Glavina Del Rio; Hope Tice; Jan-Fang Cheng; Sam Pitluck; Konstantinos Liolios; Natalia Ivanova; Roxanne Tapia; Cliff Han; David Bruce; Lynne Goodwin; Amrita Pati; Ami Chen; Krishna Palaniappan; Miriam Land; Loren Hauser; Yun-Juan Chang; Cynthia D Jeffries; John C Detter; Manfred Rohde; Evelyne Brambilla; Stefan Spring; Markus Göker; Johannes Sikorski; Tanja Woyke; James Bristow; Jonathan A Eisen; Victor Markowitz; Philip Hugenholtz; Hans-Peter Klenk; Nikos C Kyrpides
Journal:  Stand Genomic Sci       Date:  2010-10-18
  4 in total

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