Literature DB >> 3311031

Nitrogenase of Klebsiella pneumoniae. Rhodanese-catalysed restoration of activity of the inactive 2Fe species of the Fe protein.

S Pagani1, M Eldridge, R R Eady.   

Abstract

The inactive 2Fe species of the Fe protein of the nitrogenase of Klebsiella pneumoniae was generated by treating oxidized Fe protein (Kp2) with MgATP and chelator. Incubation of the 2Fe species of Kp2 with the sulphurtransferase rhodanese in the presence of thiosulphate, ferric citrate and reduced lipoate reproducibly restored activity. The extent of restoration of activity depended on the molar ratio of 2Fe Kp2 to rhodanese and was time-dependent. Re-activation did not occur in the reaction mixture lacking rhodanese.

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Year:  1987        PMID： 3311031      PMCID： PMC1148016          DOI： 10.1042/bj2440485

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

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Authors:  G L ELLMAN
Journal:  Arch Biochem Biophys       Date:  1959-05       Impact factor: 4.013

2.  Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.

Authors:  G P Roberts; T MacNeil; D MacNeil; W J Brill
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

3.  Liberation of labile sulfur from ferredoxins by alkaline zinc reagent: an appraisal of the methylene blue method.

Authors:  L Timcenko; T Kimura
Journal:  Anal Biochem       Date:  1979-06       Impact factor: 3.365

4.  Effect of magnesium adenosine 5'-triphosphate on the accessibility of the iron of clostridial azoferredoxin, a component of nitrogenase.

Authors:  G A Walker; L E Mortenson
Journal:  Biochemistry       Date:  1974-05-21       Impact factor: 3.162

5.  Crystalline rhodanese from beef kidney.

Authors:  C Cannella; L Pecci; G Federici
Journal:  Ital J Biochem       Date:  1972 Jan-Apr

6.  The mechanism of the rhodanese-catalyzed thiosulfate-lipoate reaction. Kinetic analysis.

Authors:  M Volini; J Westley
Journal:  J Biol Chem       Date:  1966-11-25       Impact factor: 5.157

7.  Structural studies of bovine liver rhodanese. I. Isolation and characterization of two active forms of the enzyme.

Authors:  K M Blumenthal; R L Heinrikson
Journal:  J Biol Chem       Date:  1971-04-25       Impact factor: 5.157

8.  Rhodanese-Mediated sulfur transfer to succinate dehydrogenase.

Authors:  F Bonomi; S Pagani; P Cerletti; C Cannella
Journal:  Eur J Biochem       Date:  1977-01-03

9.  Nitrogenase: the reaction between the Fe protein and bathophenanthrolinedisulfonate as a probe for interactions with MgATP.

Authors:  T Ljones; R H Burris
Journal:  Biochemistry       Date:  1978-05-16       Impact factor: 3.162

10.  The inhibition of rhodanese by lipoate and iron-sulfur proteins.

Authors:  S Pagani; F Bonomi; P Cerletti
Journal:  Biochim Biophys Acta       Date:  1983-01-12
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  7 in total

1.  Improved Assay for Rhodanese in Thiobacillus spp.

Authors:  D R Singleton; D W Smith
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

Review 2.  Sulphane sulphur in biological systems: a possible regulatory role.

Authors:  J I Toohey
Journal:  Biochem J       Date:  1989-12-15       Impact factor: 3.857

3.  Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii.

Authors:  M R Jacobson; V L Cash; M C Weiss; N F Laird; W E Newton; D R Dean
Journal:  Mol Gen Genet       Date:  1989-10

4.  Molybdenum trafficking for nitrogen fixation.

Authors:  Jose A Hernandez; Simon J George; Luis M Rubio
Journal:  Biochemistry       Date:  2009-10-20       Impact factor: 3.162

5.  Nucleotide sequence and genetic analysis of the Azotobacter chroococcum nifUSVWZM gene cluster, including a new gene (nifP) which encodes a serine acetyltransferase.

Authors:  D J Evans; R Jones; P R Woodley; J R Wilborn; R L Robson
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

6.  The nifU, nifS and nifV gene products are required for activity of all three nitrogenases of Azotobacter vinelandii.

Authors:  C Kennedy; D Dean
Journal:  Mol Gen Genet       Date:  1992-02

7.  A conserved mechanism for sulfonucleotide reduction.

Authors:  Kate S Carroll; Hong Gao; Huiyi Chen; C David Stout; Julie A Leary; Carolyn R Bertozzi
Journal:  PLoS Biol       Date:  2005-07-19       Impact factor: 8.029
  7 in total

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