Literature DB >> 16347513

Cloning of the Pachysolen tannophilus Xylulokinase Gene by Complementation in Escherichia coli.

P E Stevis1, J J Huang, N W Ho.   

Abstract

The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Saccharomyces cerevisiae xylulokinase genes.

Entities:  

Year:  1987        PMID： 16347513      PMCID： PMC204233          DOI： 10.1128/aem.53.12.2975-2977.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  Identification of chromosomal location of yeast DNA from hybrid plasmid p Yeleu 10.

Authors:  J Hicks; G R Fink
Journal:  Nature       Date:  1977-09-15       Impact factor: 49.962

2.  Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors:  J R Broach; J N Strathern; J B Hicks
Journal:  Gene       Date:  1979-12       Impact factor: 3.688

3.  Control of xylose metabolism in Escherichia coli.

Authors:  J D David; H Wiesmeyer
Journal:  Biochim Biophys Acta       Date:  1970-03-24

4.  Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli.

Authors:  V B Lawlis; M S Dennis; E Y Chen; D H Smith; D J Henner
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

5.  Uptake and catabolism of D-xylose in Salmonella typhimurium LT2.

Authors:  D K Shamanna; K E Sanderson
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

6.  New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.

Authors:  J C Way; M A Davis; D Morisato; D E Roberts; N Kleckner
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

7.  Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

Authors:  G Tschumper; J Carbon
Journal:  Gene       Date:  1980-07       Impact factor: 3.688

8.  Cloning and characterization of the xyl genes from Escherichia coli.

Authors:  S A Rosenfeld; P E Stevis; N W Ho
Journal:  Mol Gen Genet       Date:  1984
  8 in total
  3 in total

1.  Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.

Authors:  Rongliang Wang; Ling Zhang; Dongmei Wang; Xiaolian Gao; Jiong Hong
Journal:  J Ind Microbiol Biotechnol       Date:  2011-03-31       Impact factor: 3.346

2.  Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function.

Authors:  Yong-Su Jin; Sharon Jones; Nian-Qing Shi; Thomas W Jeffries
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

3.  Ethanolic fermentation of pentoses in lignocellulose hydrolysates.

Authors:  B Hahn-Hägerdal; T Lindén; T Senac; K Skoog
Journal:  Appl Biochem Biotechnol       Date:  1991       Impact factor: 2.926
  3 in total

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