Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning and characterization of the xyl genes from Escherichia coli.

Literature DB >> 6330500

Cloning and characterization of the xyl genes from Escherichia coli.

Abstract

Specific xylose utilization mutants of Escherichia coli were isolated that had altered xylose isomerase ( xylA ), xylulokinase ( xylB ), and regulatory ( xylR ) or transport ( xylT ) activities. We screened the Clarke and Carbon E. coli gene bank and one clone, pLC10 -15, was found to complement the xyl mutants we had characterized. Subcloning and DNA restriction mapping allowed us to locate the xylA and xylB genes on a 1.6 kbp Bg/II fragment and a 2.6 kbp HindIII-Sa/I fragment, respectively. The identification and mapping of xyl gene promoters suggest that the xylA and xylB genes are organized as an operon having a single xylose inducible promoter preceding the xylA gene.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1984 PMID： 6330500 DOI： 10.1007/bf00425552

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

20 in total

1. Genetics and regulation of D-xylose utilization in Salmonella typhimurium LT2.

Authors: D K Shamanna; K E Sanderson
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

2. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

Authors: G N Godson; D Vapnek
Journal: Biochim Biophys Acta Date: 1973-04-11

3. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

4. Control of xylose metabolism in Escherichia coli.

Authors: J D David; H Wiesmeyer
Journal: Biochim Biophys Acta Date: 1970-03-24

5. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors: L Clarke; J Carbon
Journal: Cell Date: 1976-09 Impact factor: 41.582

6. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.

Authors: N L Lee; W O Gielow; R G Wallace
Journal: Proc Natl Acad Sci U S A Date: 1981-02 Impact factor: 11.205

7. The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.

Authors: S Ogden; D Haggerty; C M Stoner; D Kolodrubetz; R Schleif
Journal: Proc Natl Acad Sci U S A Date: 1980-06 Impact factor: 11.205

8. A Col E1 hybrid plasmid containing Escherichia coli genes complementing d-xylose negative mutants of Escherichia coli and Salmonella typhimurium.

Authors: R Maleszka; P Y Wang; H Schneider
Journal: Can J Biochem Date: 1982-02

7. Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator.

Authors: S Song; C Park
Journal: J Bacteriol Date: 1997-11 Impact factor: 3.490

Cloning and characterization of the xyl genes from Escherichia coli.

1. Genetics and regulation of D-xylose utilization in Salmonella typhimurium LT2.

2. A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

3. General method for the isolation of plasmid deoxyribonucleic acid.

4. Control of xylose metabolism in Escherichia coli.

5. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

6. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.

7. The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.

8. A Col E1 hybrid plasmid containing Escherichia coli genes complementing d-xylose negative mutants of Escherichia coli and Salmonella typhimurium.

9. Factors affecting the transformation of Escherichia coli strain chi1776 by pBR322 plasmid DNA.

10. Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes.

1. Construction of stable, single-copy luciferase gene fusions in Escherichia coli.

2. Cloning of the Pachysolen tannophilus Xylulokinase Gene by Complementation in Escherichia coli.

Review 3. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

4. Topology of RbsC, a membrane component of the ribose transporter, belonging to the AraH superfamily.

Review 5. Linkage map of Escherichia coli K-12, edition 8.

6. Cloning the yeast xylulokinase gene for the improvement of xylose fermentation. Scientific note.

7. Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator.

8. Cloning and improving the expression of Pichia stipitis xylose reductase gene in Saccharomyces cerevisiae.

9. Genetic organization and regulation of the xylose degradation genes in Streptomyces rubiginosus.

Review 10. Functions of the gene products of Escherichia coli.