Literature DB >> 3896936

Cloning of the pectate lyase genes from Erwinia carotovora and their expression in Escherichia coli.

S P Lei, H C Lin, L Heffernan, G Wilcox.   

Abstract

A hybrid cosmid coding for pectate lyase (PL) activity was identified from an Erwinia carotovora genomic library by an immunological screening method. A 7-kb DNA fragment was identified which codes for three proteins identical in size to proteins with PL activity purified from E. carotovora culture supernatants. The three proteins had apparent Mrs of 41, 44 and 44 X 10(3) as estimated by SDS-PAGE. None of the PLs were exported from Escherichia coli strain HB101 but all were found in the periplasmic space. Plant tissue was macerated by the PLs made in E. coli.

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Year:  1985        PMID： 3896936     DOI： 10.1016/0378-1119(85)90158-1

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  14 in total

1.  A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity.

Authors:  L Ward; S H de Boer
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

2.  Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot.

Authors:  J M Costa; J E Loper
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792

Review 3.  The complete general secretory pathway in gram-negative bacteria.

Authors:  A P Pugsley
Journal:  Microbiol Rev       Date:  1993-03

4.  Characterization of the Erwinia carotovora pelB gene and its product pectate lyase.

Authors:  S P Lei; H C Lin; S S Wang; J Callaway; G Wilcox
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

5.  Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli.

Authors:  N T Keen; S Tamaki
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

6.  araB Gene and nucleotide sequence of the araC gene of Erwinia carotovora.

Authors:  S P Lei; H C Lin; L Heffernan; G Wilcox
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

7.  Regulation of the production of extracellular pectinase, cellulase, and protease in the soft rot bacterium Erwinia carotovora subsp. carotovora: evidence that aepH of E. carotovora subsp. carotovora 71 activates gene expression in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli.

Authors:  H Murata; A Chatterjee; Y Liu; A K Chatterjee
Journal:  Appl Environ Microbiol       Date:  1994-09       Impact factor: 4.792

8.  Identification of a global repressor gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp.

Authors:  Y Cui; A Chatterjee; Y Liu; C K Dumenyo; A K Chatterjee
Journal:  J Bacteriol       Date:  1995-09       Impact factor: 3.490

9.  Molecular cloning and sequencing of a pectate lyase gene from Yersinia pseudotuberculosis.

Authors:  S Manulis; D Y Kobayashi; N T Keen
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

10.  Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production.

Authors:  H T Saarilahti; M Pirhonen; M B Karlsson; D Flego; E T Palva
Journal:  Mol Gen Genet       Date:  1992-07
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