Literature DB >> 16346509

Properties of alginate lyases from marine bacteria.

R S Doubet1, R S Quatrano.   

Abstract

Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes.

Entities:  

Year:  1984        PMID： 16346509      PMCID： PMC239751          DOI： 10.1128/aem.47.4.699-703.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification.

Authors:  A WEISSBACH; J HURWITZ
Journal:  J Biol Chem       Date:  1959-04       Impact factor: 5.157

2.  Isolation of marine bacteria capable of producing specific lyases for alginate degradation.

Authors:  R S Doubet; R S Quatrano
Journal:  Appl Environ Microbiol       Date:  1982-09       Impact factor: 4.792

3.  Polyacrylamide gel electrophoresis of alginic acid.

Authors:  C Bucke
Journal:  J Chromatogr       Date:  1974-02-13

4.  Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay.

Authors:  J C Bearden
Journal:  Biochim Biophys Acta       Date:  1978-04-26

5.  Isolation of a unique marine bacterium capable of growth on a wide variety of polysaccharides from macroalgae.

Authors:  R S Quatrano; B A Caldwell
Journal:  Appl Environ Microbiol       Date:  1978-12       Impact factor: 4.792

6.  Alginic acid degradation by eliminases from abalone hepatopancreas.

Authors:  H I Nakada; P C Sweeny
Journal:  J Biol Chem       Date:  1967-03-10       Impact factor: 5.157

7.  Substrate specificity of endo-polyguluronide lyases from Pseudomonas sp. on the basis of their kinetic properties.

Authors:  K H Min; S Sasaki; Y Kashiwabara; K Nisizawa
Journal:  J Biochem       Date:  1977-03       Impact factor: 3.387

8.  An alginate lysate from Azotobacter vinelandii phage.

Authors:  I W Davidson; C J Lawson; I W Sutherland
Journal:  J Gen Microbiol       Date:  1977-01

9.  Cell wall assembly in fucus zygotes: I. Characterization of the polysaccharide components.

Authors:  R S Quatrano; P T Stevens
Journal:  Plant Physiol       Date:  1976-08       Impact factor: 8.340

10.  Isolation of poly-alpha-L-guluronate lyase from Klebsiella aerogenes.

Authors:  J Boyd; J R Turvey
Journal:  Carbohydr Res       Date:  1977-08       Impact factor: 2.104
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  9 in total

1.  Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity.

Authors:  P Gacesa; F S Wusteman
Journal:  Appl Environ Microbiol       Date:  1990-07       Impact factor: 4.792

2.  Purification and characterization of an alginate lyase from marine Bacterium Vibrio sp. mutant strain 510-64.

Authors:  Xiaoke Hu; Xiaolu Jiang; Huey-Min Hwang
Journal:  Curr Microbiol       Date:  2006-06-26       Impact factor: 2.188

3.  Potential commercial applications in aquatic microbiology.

Authors:  J T Staley; P M Stanley
Journal:  Microb Ecol       Date:  1986-03       Impact factor: 4.552

4.  Novel Molecular Insights into the Catalytic Mechanism of Marine Bacterial Alginate Lyase AlyGC from Polysaccharide Lyase Family 6.

Authors:  Fei Xu; Fang Dong; Peng Wang; Hai-Yan Cao; Chun-Yang Li; Ping-Yi Li; Xiu-Hua Pang; Yu-Zhong Zhang; Xiu-Lan Chen
Journal:  J Biol Chem       Date:  2017-02-01       Impact factor: 5.157

5.  Isolation and characterization of an alginate lyase from Klebsiella aerogenes.

Authors:  B Lange; J Wingender; U K Winkler
Journal:  Arch Microbiol       Date:  1989       Impact factor: 2.552

6.  Alginase enzyme production by Bacillus circulans.

Authors:  J B Hansen; R S Doubet; J Ram
Journal:  Appl Environ Microbiol       Date:  1984-04       Impact factor: 4.792

7.  Clostridium grantii sp. nov., a new obligately anaerobic, alginolytic bacterium isolated from mullet gut.

Authors:  D O Mountfort; F A Rainey; J Burghardt; E Stackebrandt
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

8.  Cloning of alginate lyase gene (alxM) and expression in Escherichia coli.

Authors:  B J Brown; J F Preston; L O Ingram
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

9.  Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis.

Authors:  W M Dunne; F L Buckmire
Journal:  Appl Environ Microbiol       Date:  1985-09       Impact factor: 4.792
  9 in total

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