Literature DB >> 16348243

Plate assay for simultaneous detection of alginate lyases and determination of substrate specificity.

P Gacesa1, F S Wusteman.   

Abstract

A plate assay to detect the presence of alginate lyases (EC 4.2.2.3) has been developed. The simultaneous use of specific alginate block structures of defined composition allows the substrate specificity of the enzymes to be determined. Clearing zones in the alginate-containing media are visualized with either cetyl pyridinium chloride or ruthenium red.

Entities:  

Year:  1990        PMID: 16348243      PMCID: PMC184597          DOI: 10.1128/aem.56.7.2265-2267.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  11 in total

1.  Bacterial alginases.

Authors:  P Gacesa; R C Caswell; P Kille
Journal:  Antibiot Chemother (1971)       Date:  1989

2.  Detection of sugars on paper chromatograms.

Authors:  W E TREVELYAN; D P PROCTER; J S HARRISON
Journal:  Nature       Date:  1950-09-09       Impact factor: 49.962

3.  Properties of alginate lyases from marine bacteria.

Authors:  R S Doubet; R S Quatrano
Journal:  Appl Environ Microbiol       Date:  1984-04       Impact factor: 4.792

4.  Kinetics and specificity of alginate lyases: Part I, A case study.

Authors:  F Haugen; F Kortner; B Larsen
Journal:  Carbohydr Res       Date:  1990-04-02       Impact factor: 2.104

5.  A method for the determination of uronic acid sequence in alginates.

Authors:  A Penman; G R Sanderson
Journal:  Carbohydr Res       Date:  1972-12       Impact factor: 2.104

6.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  Alginase enzyme production by Bacillus circulans.

Authors:  J B Hansen; R S Doubet; J Ram
Journal:  Appl Environ Microbiol       Date:  1984-04       Impact factor: 4.792

8.  Isolation of poly-alpha-L-guluronate lyase from Klebsiella aerogenes.

Authors:  J Boyd; J R Turvey
Journal:  Carbohydr Res       Date:  1977-08       Impact factor: 2.104

9.  A study of the reaction catalysed by alginate lyase VI from the sea mollusc, Littorina sp.

Authors:  V V Favorov; E I Vozhova; V A Denisenko; L A Elyakova
Journal:  Biochim Biophys Acta       Date:  1979-08-15

10.  Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis.

Authors:  W M Dunne; F L Buckmire
Journal:  Appl Environ Microbiol       Date:  1985-09       Impact factor: 4.792

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  20 in total

1.  Ulvan lyases isolated from the Flavobacteria Persicivirga ulvanivorans are the first members of a new polysaccharide lyase family.

Authors:  Pi Nyvall Collén; Jean-François Sassi; Hélène Rogniaux; Hélène Marfaing; William Helbert
Journal:  J Biol Chem       Date:  2011-10-18       Impact factor: 5.157

2.  Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2.

Authors:  Hitoshi Kawamoto; Akio Horibe; Yasunari Miki; Takayuki Kimura; Katsunori Tanaka; Tsuyoshi Nakagawa; Makoto Kawamukai; Hideyuki Matsuda
Journal:  Mar Biotechnol (NY)       Date:  2006-07-03       Impact factor: 3.619

3.  Exploiting fine-scale genetic and physiological variation of closely related microbes to reveal unknown enzyme functions.

Authors:  Ahmet H Badur; Matthew J Plutz; Geethika Yalamanchili; Sujit Sadashiv Jagtap; Thomas Schweder; Frank Unfried; Stephanie Markert; Martin F Polz; Jan-Hendrik Hehemann; Christopher V Rao
Journal:  J Biol Chem       Date:  2017-06-07       Impact factor: 5.157

4.  Structure and Polymannuronate Specificity of a Eukaryotic Member of Polysaccharide Lyase Family 14.

Authors:  Hui-Min Qin; Takuya Miyakawa; Akira Inoue; Ryuji Nishiyama; Akira Nakamura; Atsuko Asano; Yoriko Sawano; Takao Ojima; Masaru Tanokura
Journal:  J Biol Chem       Date:  2016-12-23       Impact factor: 5.157

5.  Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability.

Authors:  Akira Inoue; Moe Anraku; Satoshi Nakagawa; Takao Ojima
Journal:  J Biol Chem       Date:  2016-05-26       Impact factor: 5.157

6.  A polysaccharide lyase from Stenotrophomonas maltophilia with a unique, pH-regulated substrate specificity.

Authors:  Logan C MacDonald; Bryan W Berger
Journal:  J Biol Chem       Date:  2013-11-20       Impact factor: 5.157

7.  Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci.

Authors:  Cong Sun; Ge-Yi Fu; Chong-Ya Zhang; Jing Hu; Lin Xu; Rui-Jun Wang; Yue Su; Shuai-Bo Han; Xiao-Yun Yu; Hong Cheng; Xin-Qi Zhang; Ying-Yi Huo; Xue-Wei Xu; Min Wu
Journal:  Appl Environ Microbiol       Date:  2016-05-02       Impact factor: 4.792

8.  Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme.

Authors:  A Peciña; A Pascual; A Paneque
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

9.  Characterization of Microbulbifer strain CMC-5, a new biochemical variant of Microbulbifer elongatus type strain DSM6810T isolated from decomposing seaweeds.

Authors:  RaviChand Jonnadula; Pankaj Verma; Yogesh S Shouche; Sanjeev C Ghadi
Journal:  Curr Microbiol       Date:  2009-12       Impact factor: 2.188

10.  Copper as a signal for alginate synthesis in Pseudomonas syringae pv. syringae.

Authors:  S P Kidambi; G W Sundin; D A Palmer; A M Chakrabarty; C L Bender
Journal:  Appl Environ Microbiol       Date:  1995-06       Impact factor: 4.792

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