Literature DB >> 647023

Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay.

J C Bearden.   

Abstract

A quantitative assay method for protein is described which is based on the color change occurring in Coomassie Brilliant Blue G-250 when it binds to protein. This modification of two similar procedures further increases the sensitivity, simplicity, and stability of the protein-dye binding assay over those of other commonly used assays for protein.

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Year:  1978        PMID： 647023     DOI： 10.1016/0005-2795(78)90398-7

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  68 in total

1.  Purification and characterization of a dimer form of the cAMP-dependent protein kinase from mouse liver cytosol.

Authors:  E Nikolakaki; A Fissentzidis; T Giannakouros; J G Georgatsos
Journal:  Mol Cell Biochem       Date:  1999-07       Impact factor: 3.396

2.  Purification of the major UsnRNPs from broad bean nuclear extracts and characterization of their protein constituents.

Authors:  Z Pálfi; M Bach; F Solymosy; R Lührmann
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971

3.  Phosphorylation of Thr-948 at the C terminus of the plasma membrane H(+)-ATPase creates a binding site for the regulatory 14-3-3 protein.

Authors:  F Svennelid; A Olsson; M Piotrowski; M Rosenquist; C Ottman; C Larsson; C Oecking; M Sommarin
Journal:  Plant Cell       Date:  1999-12       Impact factor: 11.277

4.  Identification of membrane-associated proteins regulated by the arbuscular mycorrhizal symbiosis.

Authors:  Benoît Valot; Marc Dieu; Ghislaine Recorbet; Martine Raes; Silvio Gianinazzi; Eliane Dumas-Gaudot
Journal:  Plant Mol Biol       Date:  2005-11       Impact factor: 4.076

5.  Plasma membrane vesicles from source and sink leaves : changes in solute transport and polypeptide composition.

Authors:  R Lemoine; O Gallet; C Gaillard; W Frommer; S Delrot
Journal:  Plant Physiol       Date:  1992-11       Impact factor: 8.340

6.  Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays.

Authors:  V Walbot; E H Coe
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

7.  Acetylene Reduction by Symbiosomes and Free Bacteroids from Broad Bean (Vicia faba L.) Nodules (Role of Oxalate).

Authors:  J. C. Trinchant; V. Guerin; J. Rigaud
Journal:  Plant Physiol       Date:  1994-06       Impact factor: 8.340

8.  Polyphosphoinositide Phospholipase C in Plasma Membranes of Wheat (Triticum aestivum L.) : Orientation of Active Site and Activation by Ca and Mg.

Authors:  C Pical; A S Sandelius; P M Melin; M Sommarin
Journal:  Plant Physiol       Date:  1992-11       Impact factor: 8.340

9.  Juvenile hormone receptors in insect larval epidermis: identification by photoaffinity labeling.

Authors:  S R Palli; E O Osir; W Eng; M F Boehm; M Edwards; P Kulcsar; I Ujvary; K Hiruma; G D Prestwich; L M Riddiford
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

10.  Transformation of estrone and estradiol in hormone-dependent and hormone-independent human breast cancer cells. Effects of the antiestrogen ICI 164,384, danazol, and promegestone (R-5020).

Authors:  B L Nguyen; G Chetrite; J R Pasqualini
Journal:  Breast Cancer Res Treat       Date:  1995-05       Impact factor: 4.872
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