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Literature DB >> 16346464

Genetic Construction of Lactose-Utilizing Xanthomonas campestris.

Abstract

Xanthomonas campestris, the producer of xanthan gum, possesses a beta-galactosidase of very low specific activity. Plasmid pGC9114 (RP1::Tn951), generated by the transposition of the lactose transposon Tn951 to RP1, was conjugally transferred into XN1, a nalidixic acid-resistant derivative of X. campestris NRRL B-1459S-4L. Transfer occurred on membrane filters and in broth. The beta-galactosidase gene of Tn951 was expressed in X. campestris. The specific activity of beta-galactosidase in transconjugants was over 200-fold higher than that in XN1, and transconjugants grew as well in lactose-based media as in glucose-based media. The lactose-utilizing transconjugants could potentially be used to produce xanthan gum from cheese whey.

Entities: Chemical Species

Year: 1984 PMID： 16346464 PMCID： PMC239654 DOI： 10.1128/aem.47.2.253-257.1984

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

15 in total

1. General Properties of Beta-Galactosidase of Xanthomonas campestris.

Authors: J F Frank; G A Somkuti
Journal: Appl Environ Microbiol Date: 1979-09 Impact factor: 4.792

2. Nutritional Studies on Xanthan Production by Xanthomonas campestris NRRL B1459.

Authors: P Souw; A L Demain
Journal: Appl Environ Microbiol Date: 1979-06 Impact factor: 4.792

3. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules.

Authors: F L Macrina; D J Kopecko; K R Jones; D J Ayers; S M McCowen
Journal: Plasmid Date: 1978-06 Impact factor: 3.466

4. Tn951: a new transposon carrying a lactose operon.

Authors: G Cornelis; D Ghosal; H Saedler
Journal: Mol Gen Genet Date: 1978-04-06

5. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors: D B Clewell; D R Helinski
Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

6. Sequence relationships between plasmids carrying genes for lactose utilization.

Authors: G Cornelis
Journal: J Gen Microbiol Date: 1981-05

7. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas.

Authors: S Baumberg; G Cornelis; M Panagiotakopoulos; M Roberts
Journal: J Gen Microbiol Date: 1980-07

8. Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range.

Authors: R Meyer; R Laux; G Boch; M Hinds; R Bayly; J A Shapiro
Journal: J Bacteriol Date: 1982-10 Impact factor: 3.490

9. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

Authors: M Bagdasarian; R Lurz; B Rückert; F C Franklin; M M Bagdasarian; J Frey; K N Timmis
Journal: Gene Date: 1981-12 Impact factor: 3.688

7. Lactose consuming strains of Xanthomonas citri subsp. citri (Xcc) insight into the emergence of natural field resources for xanthan gum production.

Authors: Aghdas Ramezani; Mahyat Jafari; Tannaz Goodarzi; Seyed Mehdi Alavi; Ali Hatef Salmanian; Mehrdad Azin
Journal: World J Microbiol Biotechnol Date: 2013-12-08 Impact factor: 3.312

8. Construction of Lactose-Utilizing Xanthomonas campestris with a Mini-Mu Derivative.

Authors: H Drahovska; J Turna
Journal: Appl Environ Microbiol Date: 1995-02 Impact factor: 4.792

8 in total

Genetic Construction of Lactose-Utilizing Xanthomonas campestris.

1. General Properties of Beta-Galactosidase of Xanthomonas campestris.

2. Nutritional Studies on Xanthan Production by Xanthomonas campestris NRRL B1459.

3. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules.

4. Tn951: a new transposon carrying a lactose operon.

5. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

6. Sequence relationships between plasmids carrying genes for lactose utilization.

7. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas.

8. Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range.

9. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.

10. Properties of a R factor which originated in Pseudomonas aeruginosa 1822.

1. Use of whey for production of exocellular polysaccharide by a mutant strain of Xanthomonas campestris.

2. Production of High-Viscosity Whey-Glucose Broths by a Xanthomonas campestris Strain.

3. Genetic engineering of microorganisms for biotechnology.

4. Production of High-Viscosity Whey Broths by a Lactose-Utilizing Xanthomonas campestris Strain.

5. Construction of lactose-utilizing Xanthomonas campestris and production of xanthan gum from whey.

6. Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism.

7. Lactose consuming strains of Xanthomonas citri subsp. citri (Xcc) insight into the emergence of natural field resources for xanthan gum production.

8. Construction of Lactose-Utilizing Xanthomonas campestris with a Mini-Mu Derivative.