Literature DB >> 16346443

Analysis of Poly-beta-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection.

D B Karr1, J K Waters, D W Emerich.   

Abstract

Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-beta-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. The Aminex-HPLC method provides a rapid and simple chromatographic technique for routine analysis of organic acids. Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis.

Entities:  

Year:  1983        PMID： 16346443      PMCID： PMC239573          DOI： 10.1128/aem.46.6.1339-1344.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Determination of acetate by gas-liquid chromatography.

Authors:  M Wadke; J M Lowenstein
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Assay of poly-beta-hydroxybutyric acid.

Authors:  J H LAW; R A SLEPECKY
Journal:  J Bacteriol       Date:  1961-07       Impact factor: 3.490

3.  Polymeric Beta-Hydroxyalkanoates from Environmental Samples and Bacillus megaterium.

Authors:  R H Findlay; D C White
Journal:  Appl Environ Microbiol       Date:  1983-01       Impact factor: 4.792

4.  Purification of Poly-beta-Hydroxybutyrate by Density Gradient Centrifugation in Sodium Bromide.

Authors:  K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1982-05       Impact factor: 4.792

5.  Determination of volatile fatty acids and some related compounds in ovine rumen fluid, urine, and blood plasma, by gas-liquid chromatography.

Authors:  J P Ryan
Journal:  Anal Biochem       Date:  1980-11-01       Impact factor: 3.365

6.  Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity.

Authors:  P P Wong; H J Evans
Journal:  Plant Physiol       Date:  1971-06       Impact factor: 8.340

7.  Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

Authors:  D W Emerich; T Ruiz-Argüeso; T M Ching; H J Evans
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

8.  Accumulation of poly-beta-hydroxybutyrate in Spirulina platensis.

Authors:  J Campbell; S E Stevens; D L Balkwill
Journal:  J Bacteriol       Date:  1982-01       Impact factor: 3.490

9.  Purification and properties of D(-)-3-hydroxybutyrate-dimer hydrolase from Zoogloea ramigera I-16-M.

Authors:  Y Tanaka; T Saito; T Fukui; T Tanio; K Tomita
Journal:  Eur J Biochem       Date:  1981-08

10.  Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate.

Authors:  A G Ostle; J G Holt
Journal:  Appl Environ Microbiol       Date:  1982-07       Impact factor: 4.792
  10 in total
  70 in total

1.  Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16.

Authors:  Charles F Budde; Alison E Mahan; Jingnan Lu; Chokyun Rha; Anthony J Sinskey
Journal:  J Bacteriol       Date:  2010-08-20       Impact factor: 3.490

2.  Proof for a nonproteinaceous calcium-selective channel in Escherichia coli by total synthesis from (R)-3-hydroxybutanoic acid and inorganic polyphosphate.

Authors:  S Das; U D Lengweiler; D Seebach; R N Reusch
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-19       Impact factor: 11.205

3.  A large, voltage-dependent channel, isolated from mitochondria by water-free chloroform extraction.

Authors:  Evgeny Pavlov; Eleonora Zakharian; Christopher Bladen; Catherine T M Diao; Chelsey Grimbly; Rosetta N Reusch; Robert J French
Journal:  Biophys J       Date:  2005-02-04       Impact factor: 4.033

4.  Recalcitrant high-molecular-weight material, an inhibitor of microbial metabolism in river biofilms.

Authors:  C Freeman; M A Lock
Journal:  Appl Environ Microbiol       Date:  1992-06       Impact factor: 4.792

5.  Integrated recombinant protein expression and purification platform based on Ralstonia eutropha.

Authors:  Gavin C Barnard; Jesse D McCool; David W Wood; Tillman U Gerngross
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

6.  Acetate-Activating Enzymes of Bradyrhizobium japonicum Bacteroids.

Authors:  G G Preston; C Zeiher; J D Wall; D W Emerich
Journal:  Appl Environ Microbiol       Date:  1989-01       Impact factor: 4.792

7.  Tissue-specific and developmental regulation of cotton gene FbL2A. Demonstration of promoter activity in transgenic plants.

Authors:  J A Rinehart; M W Petersen; M E John
Journal:  Plant Physiol       Date:  1996-11       Impact factor: 8.340

8.  Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo.

Authors:  Mimi Cho; Christopher J Brigham; Anthony J Sinskey; JoAnne Stubbe
Journal:  Biochemistry       Date:  2012-03-07       Impact factor: 3.162

9.  Azotobacter vinelandii lacking the Na(+)-NQR activity: a potential source for producing alginates with improved properties and at high yield.

Authors:  Itzel Gaytán; Carlos Peña; Cinthia Núñez; María S Córdova; Guadalupe Espín; Enrique Galindo
Journal:  World J Microbiol Biotechnol       Date:  2012-06-07       Impact factor: 3.312

10.  New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.

Authors:  G M York; J Stubbe; A J Sinskey
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490
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