Literature DB >> 6181737

Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate.

A G Ostle, J G Holt.   

Abstract

Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.

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Year:  1982        PMID： 6181737      PMCID： PMC241995          DOI： 10.1128/aem.44.1.238-241.1982

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

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