Literature DB >> 16335973

A method for the rapid and efficient elution of native affinity-purified protein A tagged complexes.

Caterina Strambio-de-Castillia1, Jaclyn Tetenbaum-Novatt, Brian S Imai, Brian T Chait, Michael P Rout.   

Abstract

A problem faced in proteomics studies is the recovery of tagged protein complexes in their native and active form. Here we describe a peptide, Bio-Ox, that mimics the immunoglobulin G (IgG) binding interface of Staphylococcus aureus Protein A, and competitively displaces affinity-purified Protein A fusion proteins and protein complexes from IgG-Sepharose. We show that Bio-Ox elution is a robust method for the efficient and rapid recovery of native tagged proteins, and can be applied to a variety of structural genomics and proteomics studies.

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Year:  2005        PMID： 16335973     DOI： 10.1021/pr0501517

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  10 in total

1.  Saccharomyces cerevisiae Yta7 regulates histone gene expression.

Authors:  Angeline Gradolatto; Richard S Rogers; Heather Lavender; Sean D Taverna; C David Allis; John D Aitchison; Alan J Tackett
Journal:  Genetics       Date:  2008-05       Impact factor: 4.562

2.  Effects of the Fc-III tag on activity and stability of green fluorescent protein and human muscle creatine kinase.

Authors:  Shan Feng; Yiyi Gong; Gulishana Adilijiang; Haiteng Deng
Journal:  Protein Sci       Date:  2013-07       Impact factor: 6.725

3.  A pipeline for determining protein-protein interactions and proximities in the cellular milieu.

Authors:  Roman I Subbotin; Brian T Chait
Journal:  Mol Cell Proteomics       Date:  2014-08-29       Impact factor: 5.911

4.  Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.

Authors:  John LaCava; Kelly R Molloy; Martin S Taylor; Michal Domanski; Brian T Chait; Michael P Rout
Journal:  Biotechniques       Date:  2015-03-01       Impact factor: 1.993

5.  Improved native isolation of endogenous Protein A-tagged protein complexes.

Authors:  John LaCava; Nagarajan Chandramouli; Hua Jiang; Michael P Rout
Journal:  Biotechniques       Date:  2013-04       Impact factor: 1.993

Review 6.  Proteomics-based methods for discovery, quantification, and validation of protein-protein interactions.

Authors:  Yana V Miteva; Hanna G Budayeva; Ileana M Cristea
Journal:  Anal Chem       Date:  2012-12-12       Impact factor: 6.986

Review 7.  Overview of affinity tags for protein purification.

Authors:  Michelle E Kimple; Allison L Brill; Renee L Pasker
Journal:  Curr Protoc Protein Sci       Date:  2013-09-24

8.  A Robust Workflow for Native Mass Spectrometric Analysis of Affinity-Isolated Endogenous Protein Assemblies.

Authors:  Paul Dominic B Olinares; Amelia D Dunn; Júlio C Padovan; Javier Fernandez-Martinez; Michael P Rout; Brian T Chait
Journal:  Anal Chem       Date:  2016-02-18       Impact factor: 6.986

9.  Development of the Fc-III tagged protein expression system for protein purification and detection.

Authors:  Shan Feng; Enbing Tian; Lei Zhang; Qingtao Wang; Haiteng Deng
Journal:  PLoS One       Date:  2012-08-29       Impact factor: 3.240

10.  Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label.

Authors:  Yi Yang; Haiping Song; Dan He; Shuai Zhang; Shizhong Dai; Shixian Lin; Rong Meng; Chu Wang; Peng R Chen
Journal:  Nat Commun       Date:  2016-07-27       Impact factor: 14.919
  10 in total

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