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Literature DB >> 24510596

Overview of affinity tags for protein purification.

Michelle E Kimple¹, Allison L Brill¹, Renee L Pasker¹.

Abstract

Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, as well as for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.

Entities: Chemical Disease Gene Species

Keywords: affinity chromatography; affinity tag; epitope tag; protein detection; protein purification

Mesh：

Substances：
Recombinant Proteins

Year: 2013 PMID： 24510596 PMCID： PMC4527311 DOI： 10.1002/0471140864.ps0909s73

Source DB: PubMed Journal: Curr Protoc Protein Sci ISSN： 1934-3655

45 in total

1. BIA/MS of epitope-tagged peptides directly from E. coli lysate: multiplex detection and protein identification at low-femtomole to subfemtomole levels.

Authors: R W Nelson; J W Jarvik; B E Taillon; K A Tubbs
Journal: Anal Chem Date: 1999-07-15 Impact factor: 6.986

2. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.

Authors: S Chong; F B Mersha; D G Comb; M E Scott; D Landry; L M Vence; F B Perler; J Benner; R B Kucera; C A Hirvonen; J J Pelletier; H Paulus; M Q Xu
Journal: Gene Date: 1997-06-19 Impact factor: 3.688

3. Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification.

Authors: Biao Ruan; Kathryn E Fisher; Patrick A Alexander; Viktoriya Doroshko; Philip N Bryan
Journal: Biochemistry Date: 2004-11-23 Impact factor: 3.162

4. Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.

Authors: J E Cronan
Journal: J Biol Chem Date: 1990-06-25 Impact factor: 5.157

Review 5. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins.

Authors: J Nilsson; S Ståhl; J Lundeberg; M Uhlén; P A Nygren
Journal: Protein Expr Purif Date: 1997-10 Impact factor: 1.650

6. Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin.

Authors: M Hollinshead; J Sanderson; D J Vaux
Journal: J Histochem Cytochem Date: 1997-08 Impact factor: 2.479

7. Heat-mediated activation of affinity-immobilized Taq DNA polymerase.

Authors: J Nilsson; M Bosnes; F Larsen; P A Nygren; M Uhlén; J Lundeberg
Journal: Biotechniques Date: 1997-04 Impact factor: 1.993

8. Immobilization and affinity purification of recombinant proteins using histidine peptide fusions.

Authors: C Ljungquist; A Breitholtz; H Brink-Nilsson; T Moks; M Uhlén; B Nilsson
Journal: Eur J Biochem Date: 1989-12-22

Review 9. Genetic engineering of streptavidin, a versatile affinity tag.

Authors: T Sano; S Vajda; C R Cantor
Journal: J Chromatogr B Biomed Sci Appl Date: 1998-09-11

Review 10. Strategies for achieving high-level expression of genes in Escherichia coli.

Authors: S C Makrides
Journal: Microbiol Rev Date: 1996-09

51 in total

1. Unusual Stability of Anabaena Sensory Rhodopsin Transducer from Anabaena PCC7120.

Authors: Vishwa D Trivedi; Tashmay S Jones; Renee P Walker
Journal: Int J Eng Sci (Ghaziabad) Date: 2017-08-19

2. Efficient, ultra-high-affinity chromatography in a one-step purification of complex proteins.

Authors: Marina N Vassylyeva; Sergiy Klyuyev; Alexey D Vassylyev; Hunter Wesson; Zhuo Zhang; Matthew B Renfrow; Hengbin Wang; N Patrick Higgins; Louise T Chow; Dmitry G Vassylyev
Journal: Proc Natl Acad Sci U S A Date: 2017-06-12 Impact factor: 11.205

3. Protein purification using PDZ affinity chromatography.

Authors: Ward G Walkup; Mary B Kennedy
Journal: Curr Protoc Protein Sci Date: 2015-04-01

4. Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability.

Authors: Luuk Mestrom; Stefan R Marsden; Marit Dieters; Puck Achterberg; Lysanne Stolk; Isabel Bento; Ulf Hanefeld; Peter-Leon Hagedoorn
Journal: Appl Environ Microbiol Date: 2019-04-04 Impact factor: 4.792

5. Characterization of an anti-FLAG antibody binding protein in V. cholerae.

Authors: Jung-Ho Shin; Michael Lanz; Marcus B Smolka; Tobias Dörr
Journal: Biochem Biophys Res Commun Date: 2020-06-03 Impact factor: 3.575

6. Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors: Jian Xiong; Jingquan He; Wendy P Xie; Ezekiel Hinojosa; Chandra Shekar R Ambati; Nagireddy Putluri; Hyun-Eui Kim; Michael X Zhu; Guangwei Du
Journal: J Cell Sci Date: 2019-12-13 Impact factor: 5.285

7. pMINERVA: A donor-acceptor system for the in vivo recombineering of scFv into IgG molecules.

Authors: M Batonick; M M Kiss; E P Fuller; C M Magadan; E G Holland; Q Zhao; D Wang; B K Kay; M P Weiner
Journal: J Immunol Methods Date: 2016-02-03 Impact factor: 2.303

8. Application of Strep-Tactin XT for affinity purification of Twin-Strep-tagged CB₂, a G protein-coupled cannabinoid receptor.

Authors: Alexei Yeliseev; Lioudmila Zoubak; Thomas G M Schmidt
Journal: Protein Expr Purif Date: 2016-11-17 Impact factor: 1.650

9. Characterizing the supercomplex association of photosynthetic complexes in cyanobacteria.

Authors: Zimeng Zhang; Long-Sheng Zhao; Lu-Ning Liu
Journal: R Soc Open Sci Date: 2021-07-14 Impact factor: 2.963

10. Incorporation of short, charged peptide tags affects the temperature responsiveness of positively-charged elastin-like polypeptides.

Authors: Charng-Yu Lin; Julie C Liu
Journal: J Mater Chem B Date: 2019-08-06 Impact factor: 6.331