Literature DB >> 16333115

Evaluation of the LightCycler Staphylococcus M GRADE kits on positive blood cultures that contained gram-positive cocci in clusters.

Nabin K Shrestha1, Marion J Tuohy, Ravindran A Padmanabhan, Gerri S Hall, Gary W Procop.   

Abstract

We evaluated the Roche LightCycler Staphylococcus M(GRADE) kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.

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Year:  2005        PMID： 16333115      PMCID： PMC1317217          DOI： 10.1128/JCM.43.12.6144-6146.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR.

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Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

2.  Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene.

Authors:  C Y Turenne; E Witwicki; D J Hoban; J A Karlowsky; A M Kabani
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

3.  Real-time PCR can rapidly detect methicillin-susceptible and methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles.

Authors:  Suzanne M Paule; Anna C Pasquariello; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  Am J Clin Pathol       Date:  2005-09       Impact factor: 2.493

4.  Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles.

Authors:  P Lem; J Spiegelman; B Toye; K Ramotar
Journal:  Diagn Microbiol Infect Dis       Date:  2001-11       Impact factor: 2.803

5.  Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction.

Authors:  K C Carroll; R B Leonard; P L Newcomb-Gayman; D R Hillyard
Journal:  Am J Clin Pathol       Date:  1996-11       Impact factor: 2.493

6.  Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors:  T Y Tan; S Corden; R Barnes; B Cookson
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

7.  Rapid detection and identification of Staphylococcus aureus from blood culture specimens using real-time fluorescence PCR.

Authors:  Concepción Palomares; María J Torres; Antonio Torres; Javier Aznar; José C Palomares
Journal:  Diagn Microbiol Infect Dis       Date:  2003-03       Impact factor: 2.803

8.  Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

Authors:  Kenneth Oliveira; Gary W Procop; Deborah Wilson; James Coull; Henrik Stender
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

9.  Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system.

Authors:  Nabin K Shrestha; Marion J Tuohy; Gerri S Hall; Carlos M Isada; Gary W Procop
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

10.  Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes.

Authors:  Nele Wellinghausen; Beate Wirths; Axel R Franz; Lothar Karolyi; Reinhard Marre; Udo Reischl
Journal:  Diagn Microbiol Infect Dis       Date:  2004-04       Impact factor: 2.803
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Authors:  Andrea Endimiani; Kristine M Hujer; Andrea M Hujer; Sebastian Kurz; Michael R Jacobs; David S Perlin; Robert A Bonomo
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

2.  Differentiation between Staphylococcus aureus and coagulase-negative Staphylococcus species by real-time PCR including detection of methicillin resistants in comparison to conventional microbiology testing.

Authors:  Sven Klaschik; Lutz E Lehmann; Folkert Steinhagen; Malte Book; Ernst Molitor; Andreas Hoeft; Frank Stueber
Journal:  J Clin Lab Anal       Date:  2014-05-05       Impact factor: 2.352

Review 3.  Rapid diagnostics for methicillin-resistant Staphylococcus aureus: current status.

Authors:  Karen C Carroll
Journal:  Mol Diagn Ther       Date:  2008       Impact factor: 4.074

4.  Comparison of three rapid and easy bacterial DNA extraction methods for use with quantitative real-time PCR.

Authors:  S P van Tongeren; J E Degener; H J M Harmsen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-02-11       Impact factor: 3.267
  4 in total

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