Literature DB >> 16328979

Antisense-mediated inhibition of acid trehalase (ATH1) gene expression promotes ethanol fermentation and tolerance in Saccharomyces cerevisiae.

Young-Ji Jung1, Heui-Dong Park.   

Abstract

Acid trehalase gene (ATH1) expression was decreased using the antisense-RNA technique in Saccharomyces cerevisiae. The 500 bp DNA fragments containing anti-ATH1 gene between +1 and +500 were amplified using PCR and fused to yeast ADH1, CYC1 and ATH1 promoters. Yeast cells harboring the recombinant plasmids had a low activity of acid trehalase and promoted ethanol fermentation compared to the control yeast cells harboring the vector plasmid only. The recombinant yeast had a high viability with 8% (v/v) ethanol.

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Year:  2005        PMID： 16328979     DOI： 10.1007/s10529-005-3910-3

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  10 in total

1.  Expression of TPS1 gene from Saccharomycopsis fibuligera A11 in Saccharomyces sp. W0 enhances trehalose accumulation, ethanol tolerance, and ethanol production.

Authors:  Tian-Shu Cao; Zhe Chi; Guang-Lei Liu; Zhen-Ming Chi
Journal:  Mol Biotechnol       Date:  2014-01       Impact factor: 2.695

2.  Improved osmotic tolerance and ethanol production of ethanologenic Escherichia coli by IrrE, a global regulator of radiation-resistance of Deinococcus radiodurans.

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3.  Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway.

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Journal:  J Microbiol       Date:  2022-10-24       Impact factor: 2.902

4.  Improve carbon metabolic flux in Saccharomyces cerevisiae at high temperature by overexpressed TSL1 gene.

Authors:  Xiang-Yang Ge; Yan Xu; Xiang Chen
Journal:  J Ind Microbiol Biotechnol       Date:  2013-02-02       Impact factor: 3.346

Review 5.  Progress in metabolic engineering of Saccharomyces cerevisiae.

Authors:  Elke Nevoigt
Journal:  Microbiol Mol Biol Rev       Date:  2008-09       Impact factor: 11.056

6.  Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation.

Authors:  Il-Sup Kim; Young-Saeng Kim; Hyun Kim; Ingnyol Jin; Ho-Sung Yoon
Journal:  Mol Cells       Date:  2013-02-18       Impact factor: 5.034

7.  Optimization of air-blast drying process for manufacturing Saccharomyces cerevisiae and non-Saccharomyces yeast as industrial wine starters.

Authors:  Sae-Byuk Lee; Won-Seok Choi; Hyun-Jung Jo; Soo-Hwan Yeo; Heui-Dong Park
Journal:  AMB Express       Date:  2016-11-08       Impact factor: 3.298

8.  RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata.

Authors:  Olena P Ishchuk; Khadija Mohamed Ahmad; Katarina Koruza; Klara Bojanovič; Marcel Sprenger; Lydia Kasper; Sascha Brunke; Bernhard Hube; Torbjörn Säll; Thomas Hellmark; Birgitta Gullstrand; Christian Brion; Kelle Freel; Joseph Schacherer; Birgitte Regenberg; Wolfgang Knecht; Jure Piškur
Journal:  Front Microbiol       Date:  2019-07-24       Impact factor: 5.640

9.  Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae.

Authors:  Jing Zou; Xiaohui Chen; Yinghong Hu; Dongguang Xiao; Xuewu Guo; Xuedong Chang; Lisha Zhou
Journal:  Biotechnol Lett       Date:  2021-05-02       Impact factor: 2.461

10.  Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7.

Authors:  Zilong Qiu; Rongrong Jiang
Journal:  Biotechnol Biofuels       Date:  2017-05-15       Impact factor: 6.040
  10 in total

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