| Literature DB >> 16269201 |
Jason G Fewell1, Majed Matar, Gregory Slobodkin, Sang-Oh Han, Jennifer Rice, Bruce Hovanes, Danny H Lewis, Khursheed Anwer.
Abstract
The synthesis and gene delivery application of a novel lipopolymer, PEG-PEI-CHOL (PPC), is described. PPC is composed of a low molecular weight branched polyethylenimine (PEI) covalently linked with functional groups methoxypolyethyleneglycol (PEG) and cholesterol (CHOL). The potential utility of PPC as a gene delivery polymer was evaluated by showing its ability to form stable nanocomplexes with DNA, protect DNA from degradation by DNase and mediate gene transfer in vitro and in vivo in solid tumors. The ratio of PEG/PEI/CHOL and nitrogen to phosphate (Polymer/DNA) was optimized for physico-chemical properties and gene delivery efficiency of PPC/DNA complexes. The gene therapy application of the polymer was shown following administration of a murine IL-12 plasmid (pmIL-12) formulated with PPC into tumors in mice which resulted in significant inhibition of tumor growth. The inhibitory effects of pmIL-12/PPC were enhanced when combined with specific chemotherapeutic agents, demonstrating the potential usefulness of pIL-12/PPC as an adjuvant therapy for cancer treatment.Entities:
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Year: 2005 PMID： 16269201 DOI： 10.1016/j.jconrel.2005.09.024
Source DB: PubMed Journal: J Control Release ISSN： 0168-3659 Impact factor: 9.776